Hannun/Obeid Lab Protocols
Lipid Protocols
0004 Phosphate Assay by Jiandi Zhang
(JZ)
0020 DEAE Column (SJ)
0021 DEAE column: FPLC (SJ)
0022 Desalting Acidic Glycosphingolipids (SJ)
0023 Arachidonic Acid Labeling (SJ)
0024 AA & Metabolite Quantitation of Cell Pellets
Post-AA Labeling (SJ)
0025 AA & Metabolite Quantitation of Media Post-AA
Labeling (SJ)
0026 Neutralizing Arachidonic Acid (SJ)
0027 Preparation of bOG-DOPG Mixed Micelles (SJ)
0028 [3H] Choline Labeling and TNF Treatment of
HL-60 Cells (SJ)
0029 DGK Assay (SJ)
0030 DGK Membrane Preparation (SJ)
0031 Column Purification of Demethylated Sphingomyelin
(SJ)
0032 Purification of Demethylated Sphingomyelin
(SJ)
0033 Drying Lipids with Liquid Nitrogen Evaporator
(SJ)
0034 Glycosphingolipid analysis (SJ)
0035 In vitro Sphingomyelinase Assay (SJ)
0036 Labelled Sphingmyelin Synthesis (SJ)
0037 After Using the Nitrogen Evaporator (SJ)
0038 Phosphate Assay by Suprya Jayadev (SJ)
0039 Purification of Demethylated Sphingomyelin
(SJ)
0040 Sphingomyelin Quantitation Post-choline Labeling
of HL60 Cells (SJ)
0041 Sphingomyelin Mass Measurement (SJ)
0042 Thin Layer Chromatography (SJ)
Cell Biology & Cell Culture Protocols
0005 Trypsinization of Adherent Cells (SJ)
0006 Freezing Cells (SJ)
0007 Thawing cells (SJ)
0008 Selection of Transfected Suspension Cells (SJ)
0009 Apoptosis: Miniassay (SJ)
0010 Flow Cytometric Analysis of Cell Cycle (SJ)
0011 Cell Disruption and Subcellular Fractionation (SJ)
0012 Mycoplasma Testing with MycoTect Kit (SJ)
0013 NBT assay (SJ)
0014 NBT assay: Brice Weinberg protocol (SJ)
0015 Differentiation with inducers for NBT (SJ)
0016 Percoll Gradient (SJ)
0017 Proliferation Assay: [3H] Thymidine Incorporation (SJ)
0018 Preparation of TNFa (SJ)
Biochemistry Protocols
0019 Biorad Protein Assay: Bradford (SJ)
Yeast Protocols
0002 DNA Preparation from 10-mL Yeast Culture (NC)
0003 Direct PCR from Whole Yeast Cells: Zymolyase Method (NC)
0046 Yeast Strain: made with FileMakerPro 3.0 on Mac (NC)
Molecular Biology Protocols
0001 E. coli Transformation for Subcloning (NC)
0043 cDNA Synthesis from MOLT-4 Cells(CM)
0044 mRNA Purification from MOLT-4 Cells (CM)
0048 Vector List for Hannun/Obeid Lab
Computer & Miscellaneous Protocols
0045 How to Import Medline Search Result in Reference Manager (NC)
0047 3-D Structure of Sphingolipid Metabolites (NC)
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