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Daniel J. Lew
Professor of Pharmacology and Cancer Biology
Professor of Molecular Genetics and Microbiology
Director, Program in Cell and Molecular Biology
Box 3813
C359 LSRC bldg
Duke University Medical Center
Durham, NC 27710
Tel (919) 613-8627
Fax (919) 681-1005
Email daniel.lew@duke.edu
daniel.lew@duke.edu
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John Crutchley
jmc32@duke.edu
In order to complete mitosis, the cell must have a bud. How does it
sense that
a bud has formed? Our lab has determined that Hsl1p responds to the change in
cell shape that accompanies budding and then initiates Swe1p degradation, thus
allowing the cell to proceed through mitosis. I am interested in finding out
how cell shape regulates Hsl1p.
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Audrey Howell
audrey.howell@duke.edu
My work is focused on understanding how cells establish an axis of polarity. I
am using live-cell microscopy to follow the polarization of key proteins
involved in polarity establishment in a variety of genetic backgrounds.
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Jayme Johnson
jmj5@duke.edu
I am interested in how cells maintain an axis of polarity. In yeast, cells need to polarize their
growth under two conditions: budding and mating. In response to mating
pheromone, cells form a mating projection or shmoo. In order to form a
shmoo successfully, cells need to establish an axis of polarity and
maintain that axis in order to localize their growth to one site on the
cell cortex. To address the question of how cells stabilize an axis of
polarity once it is formed, I am using genetic and microscopic tools to
tease apart the roles of various proteins known to play a part in
polarity establishment.
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Kindra King
kmk18.edu
Polarized growth in budding yeast is essential for forming new buds. This new bud must be formed before the end of mitosis so that the DNA can be partitioned into the two cell bodies. If there is a problem with the bud formation, the cell will arrest in G2 at the morphogenesis checkpoint. This checkpoint is dependent on the protein Swe1. But how does Swe1p know when there is a problem? My research tries to shed some light on this question. By causing transient depolarization in cells via stress (heat, salt, sugar, ethanol), I can use genetic and microscopy methods to examine how Swe1p regulators are involved in the morphogenesis checkpoint.
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Koji Saito
koji.saito@duke.edu
I study the regulatory mechanism of Cdc24p phosphorylation. Cdc24p undergoes cell-cycle regulated hyperphosphorylation. However, how Cdc24p phosphorylation is regulated during the cell cycle and its functional consequence remain unclear. I would like to elucidate this molecular mechanism.
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Lee Szkotnicki
lee.szkotnicki@duke.edu
My project focuses on the cell cycle side of the lab where I am looking at
regulation of Swe1p, the kinase responsible for the morphogenesis checkpoint
delay. Through use of a genetic screen I identified an upstream regulator of
Swe1p. Currently I am using a variety of genetic and biochemical techniques to
determine the specific role this regulator is playing in cell cycle control.
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