LOCUS       PAG60.GB     3944 BP DS-DNA   CIRCULAR  SYN       20-JUN-2001
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
FEATURES             Location/Qualifiers
     primer_bind     complement(1539..1560)
                     /note="JM42 rev."
     promoter        110..459
                     /note="P-Tef"
     terminator      1272..1516
                     /note="T-Tef"
     gene            459..1271
                     /note="CaURA3"
     gene            complement(2617..3477)
                     /note="Amp"
     primer_bind     13..31
                     /note="JM41 fwd."
BASE COUNT      1074 A       894 C       925 G      1051 T         0 OTHER
ORIGIN      -
        1 GAACGCGGCC GCCAGCTGAA GCTTCGTACG CTGCAGGTCG ACGGATCCCC GGGTTAATTA
       61 AGGCGCGCCA GATCTGTTTA GCTTGCCTCG TCCCCGCCGG GTCACCCGGC CAGCGACATG
      121 GAGGCCCAGA ATACCCTCCT TGACAGTCTT GACGTGCGCA GCTCAGGGGC ATGATGTGAC
      181 TGTCGCCCGT ACATTTAGCC CATACATCCC CATGTATAAT CATTTGCATC CATACATTTT
      241 GATGGCCGCA CGGCGCGAAG CAAAAATTAC GGCTCCTCGC TGCAGACCTG CGAGCAGGGA
      301 AACGCTCCCC TCACAGACGC GTTGAATTGT CCCCACGCCG CGCCCCTGTA GAGAAATATA
      361 AAAGGTTAGG ATTTGCCACT GAGGTTCTTC TTTCATATAC TTCCTTTTAA AATCTTGCTA
      421 GGATACAGTT CTCACATCAC ATCCGAACAT AAACAACCAT GACAGTCAAC ACTAAGACCT
      481 ATAGTGAGAG AGCAGAAACT CATGCCTCAC CAGTAGCACA GCGATTATTT CGATTAATGG
      541 AACTGAAGAA AACCAATTTA TGTGCATCAA TTGACGTTGA TACCACTAAG GAATTCCTTG
      601 AATTAATTGA TAAATTAGGT CCTTATGTAT GCTTAATCAA GACTCATATT GATATAATCA
      661 ATGATTTTTC CTATGAATCC ACTATTGAAC CATTATTAGA ACTTTCACGT AAACATCAAT
      721 TTATGATTTT TGAAGATAGA AAATTTGCTG ATATTGGTAA TACCGTAAAG AAACAATATA
      781 TTGGTGGAGT TTATAAAATT AGTAGTTGGG CAGATATTAC CAATGCTCAT GGTGTCACTG
      841 GGAATGGAGT GGTTGAAGGA TTAAAACAGG GAGCTAAAGA AACCACCACC AACCAAGAGC
      901 CAAGAGGGTT ATTGATGTTA GCTGAATTAT CATCAGTGGG ATCATTAGCA TATGGAGAAT
      961 ATTCTCAAAA AACTGTTGAA ATTGCTAAAT CCGATAAGGA ATTTGTTATT GGATTTATTG
     1021 CCCAACGTGA TATGGGTGGC CAAGAAGAAG GATTTGATTG GCTTATTATG ACACCTGGAG
     1081 TTGGATTAGA TGATAAAGGT GATGGATTAG GACAACAATA TAGAACTGTT GATGAAGTTG
     1141 TTAGCACTGG AACTGATATT ATCATTGTTG GTAGAGGATT GTTTGGTAAA GGAAGAGATC
     1201 CAGATATTGA AGGTAAAAGG TATAGAAATG CTGGTTGGAA TGCTTATTTG AAAAAGACTG
     1261 GCCAATTATA ATCAGTACTG ACAATAAAAA GATTCTTGTT TTCAAGAACT TGTCATTTGT
     1321 ATAGTTTTTT TATATTGTAG TTGTTCTATT TTAATCAAAT GTTAGCGTGA TTTATATTTT
     1381 TTTTCGCCTC GACATCATCT GCCCAGATGC GAAGTTAAGT GCGCAGAAAG TAATATCATG
     1441 CGTCAATCGT ATGTGAATGC TGGTCGCTAT ACTGCTGTCG ATTCGATACT AACGCCGCCA
     1501 TCCAGTGTCG AAAACGAGCT CGAATTCATC GATGATATCA GATCCACTAG TGGCCTATGC
     1561 GGCCGCGGAT CTGCCGGTCT CCCTATAGTG AGTCGTATTA ATTTCGATAA GCCAGGTTAA
     1621 CCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC
     1681 CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC
     1741 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT
     1801 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT
     1861 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG
     1921 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC
     1981 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT
     2041 GGCGCTTTCT CAATGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA
     2101 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA
     2161 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA
     2221 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA
     2281 CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT
     2341 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT
     2401 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT
     2461 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT
     2521 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC
     2581 AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC
     2641 ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA
     2701 GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA
     2761 CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG
     2821 CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC
     2881 TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT
     2941 CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG
     3001 GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT
     3061 CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA
     3121 TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA
     3181 GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA
     3241 TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG
     3301 GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC
     3361 ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG
     3421 AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT
     3481 CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT
     3541 ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT
     3601 GCCACCTGAC GTCTAAGAAA CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT
     3661 CACGAGGCCC TTTCGTCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA
     3721 GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA
     3781 GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT AACTATGCGG CATCAGAGCA
     3841 GATTGTACTG AGAGTGCACC ATATGGACAT ATTGTCGTTA GAACGCGGCT ACAATTAATA
     3901 CATAACCTTA TGTATCATAC ACATACGATT TAGGTGACAC TATA
//