LOCUS       PBN50.GB     7238 BP DS-DNA   CIRCULAR  SYN       15-NOV-2006
DEFINITION  Yeast centromere vector pRS314 with TRP1 marker, complete sequence.
ACCESSION   -
KEYWORDS    -
SOURCE      Cloning vector pRS314.
REFERENCE   [1]
  AUTHORS   Sikorski, R.S. & Hieter, P.
  TITLE     A system of shuttle vectors and yeast host strains designed for
            efficient
            manipulation of DNA in Saccharomyces cerevisiae.
  JOURNAL   Genetics,  122,  1,  19-27,  (1989)
  MEDLINE   89276910
REFERENCE   [2]
  AUTHORS   Stillman, D.J.
  TITLE     Direct Submission
  JOURNAL   Submission,  (1993)
COMMENT     DT   14-SEP-1995
            Database Reference:
            GENBANK    PRS314
            Accession:  U03440
            ------------ 
            GenBank Division: SYN
FEATURES             Location/Qualifiers
     gene            468..1142
                     /note="TRP1"
     gene            join(complement(1477..>1908),complement(<4364..>4507))
                     /note="lacZa' [Split]"
     gene            complement(5664..6524)
                     /note="Amp"
     frag            1909..4363
                     /note="2013 to 4467 of pRS316+(pef/Kan/pef).MV"
     frag            1909..>4363
                     /note="9 to 2464 of pFA6-KanMX3 (with pefA DR).MV"
     primer_bind     1930..1995
                     /note="BN105"
     primer_bind     complement(4340..4361)
                     /note="JM42 rev."
     promoter        2460..>2808
                     /note="P-Tef"
     primer_bind     1913..1931
                     /note="JM41 fwd."
     terminator      3619..3858
                     /note="T-Tef"
     frag            1976..2425
                     /note="24 to 473 of pefA.MV"
     primer_bind     1976..1995
                     /note="BN103"
     primer_bind     complement(2406..2425)
                     /note="BN104"
     repeat_region   1976..2425
                     /note="pefA DR"
     frag            3859..4308
                     /note="24 to 473 of pefA.MV"
     primer_bind     3859..3878
                     /note="BN103"
     primer_bind     complement(4289..4308)
                     /note="BN104"
     repeat_region   3859..4308
                     /note="pefA DR"
     frag            2809..3618
                     /note="459 to 1268 of pFA6-kanMX4"
     misc_feature    <2809..>3618
                     /note="kanMX4 module [Split]"
     gene            2809..3618
                     /note="kanMX4"
BASE COUNT      1914 A      1640 C      1764 G      1920 T         0 OTHER
ORIGIN      Sequence 7238 BP; 1914 A; 1640 C; 1764 G; 1920 T; 0 other;
        1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
       61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
      121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
      181 ACCATAAACG ACATTACTAT ATATATAATA TAGGAAGCAT TTAATAGACA GCATCGTAAT
      241 ATATGTGTAC TTTGCAGTTA TGACGCCAGA TGGCAGTAGT GGAAGATATT CTTTATTGAA
      301 AAATAGCTTG TCACCTTACG TACAATCTTG ATCCGGAGCT TTTCTTTTTT TGCCGATTAA
      361 GAATTAATTC GGTCGAAAAA AGAAAAGGAG AGGGCCAAGA GGGAGGGCAT TGGTGACTAT
      421 TGAGCACGTG AGTATACGTG ATTAAGCACA CAAAGGCAGC TTGGAGTATG TCTGTTATTA
      481 ATTTCACAGG TAGTTCTGGT CCATTGGTGA AAGTTTGCGG CTTGCAGAGC ACAGAGGCCG
      541 CAGAATGTGC TCTAGATTCC GATGCTGACT TGCTGGGTAT TATATGTGTG CCCAATAGAA
      601 AGAGAACAAT TGACCCGGTT ATTGCAAGGA AAATTTCAAG TCTTGTAAAA GCATATAAAA
      661 ATAGTTCAGG CACTCCGAAA TACTTGGTTG GCGTGTTTCG TAATCAACCT AAGGAGGATG
      721 TTTTGGCTCT GGTCAATGAT TACGGCATTG ATATCGTCCA ACTGCATGGA GATGAGTCGT
      781 GGCAAGAATA CCAAGAGTTC CTCGGTTTGC CAGTTATTAA AAGACTCGTA TTTCCAAAAG
      841 ACTGCAACAT ACTACTCAGT GCAGCTTCAC AGAAACCTCA TTCGTTTATT CCCTTGTTTG
      901 ATTCAGAAGC AGGTGGGACA GGTGAACTTT TGGATTGGAA CTCGATTTCT GACTGGGTTG
      961 GAAGGCAAGA GAGCCCCGAA AGCTTACATT TTATGTTAGC TGGTGGACTG ACGCCAGAAA
     1021 ATGTTGGTGA TGCGCTTAGA TTAAATGGCG TTATTGGTGT TGATGTAAGC GGAGGTGTGG
     1081 AGACAAATGG TGTAAAAGAC TCTAACAAAA TAGCAAATTT CGTCAAAAAT GCTAAGAAAT
     1141 AGGTTATTAC TGAGTAGTAT TTATTTAAGT ATTGTTTGTG CACTTGCCTG CGGTGTGAAA
     1201 TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGAA ATTGTAAACG TTAATATTTT
     1261 GTTAAAATTC GCGTTAAATT TTTGTTAAAT CAGCTCATTT TTTAACCAAT AGGCCGAAAT
     1321 CGGCAAAATC CCTTATAAAT CAAAAGAATA GACCGAGATA GGGTTGAGTG TTGTTCCAGT
     1381 TTGGAACAAG AGTCCACTAT TAAAGAACGT GGACTCCAAC GTCAAAGGGC GAAAAACCGT
     1441 CTATCAGGGC GATGGCCCAC TACGTGAACC ATCACCCTAA TCAAGTTTTT TGGGGTCGAG
     1501 GTGCCGTAAA GCACTAAATC GGAACCCTAA AGGGAGCCCC CGATTTAGAG CTTGACGGGG
     1561 AAAGCCGGCG AACGTGGCGA GAAAGGAAGG GAAGAAAGCG AAAGGAGCGG GCGCTAGGGC
     1621 GCTGGCAAGT GTAGCGGTCA CGCTGCGCGT AACCACCACA CCCGCCGCGC TTAATGCGCC
     1681 GCTACAGGGC GCGTCGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC
     1741 GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAGGGG GGATGTGCTG CAAGGCGATT
     1801 AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT
     1861 GTAATACGAC TCACTATAGG GCGAATTGGA GCTCCACCGC GGTGGCGGCC GCCAGCTGAA
     1921 GCTTCGTACG CTGCAGGTCG ACGGATCCCC GGGTTAATTA AGGCGCGCCA GATCTCATTA
     1981 TTGCACTGGG TGTTCTGGGC GGGACTGCAC ACGCTGCCAA TGAAGTAACT TTCCTGGGTT
     2041 CAGTAAGCGC AACAACCTGT GACCTGACCA CTTCTGTCAA CGGCGCCGCT CAGCCGAACC
     2101 AGGTTGTTCA GCTGGGTACC GTTCAGGCTA ACCAGCCGGG TAATTTTGTT GACTTCGCCA
     2161 TGAAACCGGT TGACCCGAAT GCACAGGGTT GTGCAAATCT GGCTCAGAAA ACGGCCACTG
     2221 TTTCCTGGGC TTCTGCTGCC CTGGATGGCG AAGGCTTTGG GGCAACCAGC GGTACAGCTA
     2281 CTGATGCCAA AGTACTGGTT GAAAGCGTGA ACTCCAAAAA CCCGGGGGCT GTAAACGCTA
     2341 ACGCCAGCAC GGTTGACTTT GAAGGTGCCA AGCTGACCAC CGACGGCCTG CAGTTCAAAG
     2401 CCAAGCTGAA AGGTGGTGCG ACCGAGTTTA GCTTGCCTCG TCCCCGCCGG GTCACCCGGC
     2461 CAGCGACATG GAGGCCCAGA ATACCCTCCT TGACAGTCTT GACGTGCGCA GCTCAGGGGC
     2521 ATGATGTGAC TGTCGCCCGT ACATTTAGCC CATACATCCC CATGTATAAT CATTTGCATC
     2581 CATACATTTT GATGGCCGCA CGGCGCGAAG CAAAAATTAC GGCTCCTCGC TGCAGACCTG
     2641 CGAGCAGGGA AACGCTCCCC TCACAGACGC GTTGAATTGT CCCCACGCCG CGCCCCTGTA
     2701 GAGAAATATA AAAGGTTAGG ATTTGCCACT GAGGTTCTTC TTTCATATAC TTCCTTTTAA
     2761 AATCTTGCTA GGATACAGTT CTCACATCAC ATCCGAACAT AAACAACCAT GGGTAAGGAA
     2821 AAGACTCACG TTTCGAGGCC GCGATTAAAT TCCAACATGG ATGCTGATTT ATATGGGTAT
     2881 AAATGGGCTC GCGATAATGT CGGGCAATCA GGTGCGACAA TCTATCGATT GTATGGGAAG
     2941 CCCGATGCGC CAGAGTTGTT TCTGAAACAT GGCAAAGGTA GCGTTGCCAA TGATGTTACA
     3001 GATGAGATGG TCAGACTAAA CTGGCTGACG GAATTTATGC CTCTTCCGAC CATCAAGCAT
     3061 TTTATCCGTA CTCCTGATGA TGCATGGTTA CTCACCACTG CGATCCCCGG CAAAACAGCA
     3121 TTCCAGGTAT TAGAAGAATA TCCTGATTCA GGTGAAAATA TTGTTGATGC GCTGGCAGTG
     3181 TTCCTGCGCC GGTTGCATTC GATTCCTGTT TGTAATTGTC CTTTTAACAG CGATCGCGTA
     3241 TTTCGTCTCG CTCAGGCGCA ATCACGAATG AATAACGGTT TGGTTGATGC GAGTGATTTT
     3301 GATGACGAGC GTAATGGCTG GCCTGTTGAA CAAGTCTGGA AAGAAATGCA TAAGCTTTTG
     3361 CCATTCTCAC CGGATTCAGT CGTCACTCAT GGTGATTTCT CACTTGATAA CCTTATTTTT
     3421 GACGAGGGGA AATTAATAGG TTGTATTGAT GTTGGACGAG TCGGAATCGC AGACCGATAC
     3481 CAGGATCTTG CCATCCTATG GAACTGCCTC GGTGAGTTTT CTCCTTCATT ACAGAAACGG
     3541 CTTTTTCAAA AATATGGTAT TGATAATCCT GATATGAATA AATTGCAGTT TCATTTGATG
     3601 CTCGATGAGT TTTTCTAATC AGTACTGACA ATAAAAAGAT TCTTGTTTTC AAGAACTTGT
     3661 CATTTGTATA GTTTTTTTAT ATTGTAGTTG TTCTATTTTA ATCAAATGTT AGCGTGATTT
     3721 ATATTTTTTT TCGCCTCGAC ATCATCTGCC CAGATGCGAA GTTAAGTGCG CAGAAAGTAA
     3781 TATCATGCGT CAATCGTATG TGAATGCTGG TCGCTATACT GCTGTCGATT CGATACTAAC
     3841 GCCGCCATCC AGTGTCGACA TTATTGCACT GGGTGTTCTG GGCGGGACTG CACACGCTGC
     3901 CAATGAAGTA ACTTTCCTGG GTTCAGTAAG CGCAACAACC TGTGACCTGA CCACTTCTGT
     3961 CAACGGCGCC GCTCAGCCGA ACCAGGTTGT TCAGCTGGGT ACCGTTCAGG CTAACCAGCC
     4021 GGGTAATTTT GTTGACTTCG CCATGAAACC GGTTGACCCG AATGCACAGG GTTGTGCAAA
     4081 TCTGGCTCAG AAAACGGCCA CTGTTTCCTG GGCTTCTGCT GCCCTGGATG GCGAAGGCTT
     4141 TGGGGCAACC AGCGGTACAG CTACTGATGC CAAAGTACTG GTTGAAAGCG TGAACTCCAA
     4201 AAACCCGGGG GCTGTAAACG CTAACGCCAG CACGGTTGAC TTTGAAGGTG CCAAGCTGAC
     4261 CACCGACGGC CTGCAGTTCA AAGCCAAGCT GAAAGGTGGT GCGACCGAGT TTAAACGAGC
     4321 TCGAATTCAT CGATGATATC AGATCCACTA GTGGCCTATG CGGCCGCTCT AGAACTAGTG
     4381 GATCCCCCGG GCTGCAGGAA TTCGATATCA AGCTTATCGA TACCGTCGAC CTCGAGGGGG
     4441 GGCCCGGTAC CCAGCTTTTG TTCCCTTTAG TGAGGGTTAA TTCCGAGCTT GGCGTAATCA
     4501 TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATAGGA
     4561 GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGGTAACT CACATTAATT
     4621 GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA
     4681 ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC
     4741 ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG
     4801 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC
     4861 CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCGGC
     4921 CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA
     4981 CTATAAAGAT ACCAGGCGTT CCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC
     5041 CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAA
     5101 TGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG
     5161 CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC
     5221 AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA
     5281 GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT
     5341 AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT
     5401 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG
     5461 CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG
     5521 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA
     5581 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA
     5641 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG
     5701 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTGCCCG TCGTGTAGAT AACTACGATA
     5761 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG
     5821 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT
     5881 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT
     5941 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC
     6001 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA
     6061 TCCCCCATGT TGTGAAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT
     6121 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC
     6181 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA
     6241 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA
     6301 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA
     6361 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT
     6421 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC
     6481 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA
     6541 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT
     6601 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGGGTCC
     6661 TTTTCATCAC GTGCTATAAA AATAATTATA ATTTAAATTT TTTAATATAA ATATATAAAT
     6721 TAAAAATAGA AAGTAAAAAA AGAAATTAAA GAAAAAATAG TTTTTGTTTT CCGAAGATGT
     6781 AAAAGACTCT AGGGGGATCG CCAACAAATA CTACCTTTTA TCTTGCTCTT CCTGCTCTCA
     6841 GGTATTAATG CCGAATTGTT TCATCTTGTC TGTGTAGAAG ACCACACACG AAAATCCTGT
     6901 GATTTTACAT TTTACTTATC GTTAATCGAA TGTATATCTA TTTAATCTGC TTTTCTTGTC
     6961 TAATAAATAT ATATGTAAAG TACGCTTTTT GTTGAAATTT TTTAAACCTT TGTTTATTTT
     7021 TTTTTCTTCA TTCCGTAACT CTTCTACCTT CTTTATTTAC TTTCTAAAAT CCAAATACAA
     7081 AACATAAAAA TAAATAAACA CAGAGTAAAT TCCCAAATTA TTCCATCATT AAAAGATACG
     7141 AGGCGCGTGT AAGTTACAGG CAAGCGATCC GTCCTAAGAA ACCATTATTA TCATGACATT
     7201 AACCTATAAA AATAGGCGTA TCACGAGGCC CTTTCGTC
//