LOCUS       PBN52.GB     6951 BP DS-DNA   CIRCULAR  SYN       15-NOV-2006
DEFINITION  Yeast centromere vector pRS314 with TRP1 marker, complete sequence.
ACCESSION   -
KEYWORDS    -
SOURCE      Cloning vector pRS314.
REFERENCE   [1]
  AUTHORS   Sikorski, R.S. & Hieter, P.
  TITLE     A system of shuttle vectors and yeast host strains designed for
            efficient
            manipulation of DNA in Saccharomyces cerevisiae.
  JOURNAL   Genetics,  122,  1,  19-27,  (1989)
  MEDLINE   89276910
REFERENCE   [2]
  AUTHORS   Stillman, D.J.
  TITLE     Direct Submission
  JOURNAL   Submission,  (1993)
COMMENT     DT   14-SEP-1995
            Database Reference:
            GENBANK    PRS314
            Accession:  U03440
            ------------ 
            GenBank Division: SYN
FEATURES             Location/Qualifiers
     gene            468..1142
                     /note="TRP1"
     gene            join(complement(1477..>1908),complement(<4077..>4220))
                     /note="lacZa' [Split]"
     gene            complement(5377..6237)
                     /note="Amp"
     frag            join(1909..>2808,<3332..>4076)
                     /note="2013 to 4467 of pRS316+(pef/Kan/pef).MV [Split]"
     frag            join(1909..>2808,<3332..>4076)
                     /note="9 to 2464 of pFA6-KanMX3 (with pefA DR).MV [Split]"
     primer_bind     1930..1995
                     /note="BN105"
     primer_bind     complement(4053..4074)
                     /note="JM42 rev."
     promoter        2460..>2808
                     /note="P-Tef"
     primer_bind     1913..1931
                     /note="JM41 fwd."
     terminator      3332..3571
                     /note="T-Tef"
     frag            1976..2425
                     /note="24 to 473 of pefA.MV"
     primer_bind     1976..1995
                     /note="BN103"
     primer_bind     complement(2406..2425)
                     /note="BN104"
     repeat_region   1976..2425
                     /note="pefA DR"
     frag            3572..4021
                     /note="24 to 473 of pefA.MV"
     primer_bind     3572..3591
                     /note="BN103"
     primer_bind     complement(4002..4021)
                     /note="BN104"
     repeat_region   3572..4021
                     /note="pefA DR"
     frag            2809..3331
                     /note="2913 to 3435 of pRS316+(pef/FCA1/pef).MV"
     frag            2809..3331
                     /note="459 to 981 of pPH36 (FCA1-MX4)"
     frag            2809..3331
                     /note="858 to 1380 of C. albicans FCA1.MV"
     exon            <2809..2863
                     /note="exon"
                     /note="/number=1"
     intron          2864..2933
                     /note="intron"
     exon            2934..>3331
                     /note="exon"
                     /note="/number=2"
     mRNA            join(<2809..2863,<2934..>3331)
                     /note="cytosine deaminase"
     CDS             join(2809..2863,2934..3331)
                     /note="cytosine deaminase"
BASE COUNT      1891 A      1557 C      1673 G      1830 T         0 OTHER
ORIGIN      Sequence 6951 BP; 1891 A; 1557 C; 1673 G; 1830 T; 0 other;
        1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
       61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
      121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
      181 ACCATAAACG ACATTACTAT ATATATAATA TAGGAAGCAT TTAATAGACA GCATCGTAAT
      241 ATATGTGTAC TTTGCAGTTA TGACGCCAGA TGGCAGTAGT GGAAGATATT CTTTATTGAA
      301 AAATAGCTTG TCACCTTACG TACAATCTTG ATCCGGAGCT TTTCTTTTTT TGCCGATTAA
      361 GAATTAATTC GGTCGAAAAA AGAAAAGGAG AGGGCCAAGA GGGAGGGCAT TGGTGACTAT
      421 TGAGCACGTG AGTATACGTG ATTAAGCACA CAAAGGCAGC TTGGAGTATG TCTGTTATTA
      481 ATTTCACAGG TAGTTCTGGT CCATTGGTGA AAGTTTGCGG CTTGCAGAGC ACAGAGGCCG
      541 CAGAATGTGC TCTAGATTCC GATGCTGACT TGCTGGGTAT TATATGTGTG CCCAATAGAA
      601 AGAGAACAAT TGACCCGGTT ATTGCAAGGA AAATTTCAAG TCTTGTAAAA GCATATAAAA
      661 ATAGTTCAGG CACTCCGAAA TACTTGGTTG GCGTGTTTCG TAATCAACCT AAGGAGGATG
      721 TTTTGGCTCT GGTCAATGAT TACGGCATTG ATATCGTCCA ACTGCATGGA GATGAGTCGT
      781 GGCAAGAATA CCAAGAGTTC CTCGGTTTGC CAGTTATTAA AAGACTCGTA TTTCCAAAAG
      841 ACTGCAACAT ACTACTCAGT GCAGCTTCAC AGAAACCTCA TTCGTTTATT CCCTTGTTTG
      901 ATTCAGAAGC AGGTGGGACA GGTGAACTTT TGGATTGGAA CTCGATTTCT GACTGGGTTG
      961 GAAGGCAAGA GAGCCCCGAA AGCTTACATT TTATGTTAGC TGGTGGACTG ACGCCAGAAA
     1021 ATGTTGGTGA TGCGCTTAGA TTAAATGGCG TTATTGGTGT TGATGTAAGC GGAGGTGTGG
     1081 AGACAAATGG TGTAAAAGAC TCTAACAAAA TAGCAAATTT CGTCAAAAAT GCTAAGAAAT
     1141 AGGTTATTAC TGAGTAGTAT TTATTTAAGT ATTGTTTGTG CACTTGCCTG CGGTGTGAAA
     1201 TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGAA ATTGTAAACG TTAATATTTT
     1261 GTTAAAATTC GCGTTAAATT TTTGTTAAAT CAGCTCATTT TTTAACCAAT AGGCCGAAAT
     1321 CGGCAAAATC CCTTATAAAT CAAAAGAATA GACCGAGATA GGGTTGAGTG TTGTTCCAGT
     1381 TTGGAACAAG AGTCCACTAT TAAAGAACGT GGACTCCAAC GTCAAAGGGC GAAAAACCGT
     1441 CTATCAGGGC GATGGCCCAC TACGTGAACC ATCACCCTAA TCAAGTTTTT TGGGGTCGAG
     1501 GTGCCGTAAA GCACTAAATC GGAACCCTAA AGGGAGCCCC CGATTTAGAG CTTGACGGGG
     1561 AAAGCCGGCG AACGTGGCGA GAAAGGAAGG GAAGAAAGCG AAAGGAGCGG GCGCTAGGGC
     1621 GCTGGCAAGT GTAGCGGTCA CGCTGCGCGT AACCACCACA CCCGCCGCGC TTAATGCGCC
     1681 GCTACAGGGC GCGTCGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC
     1741 GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAGGGG GGATGTGCTG CAAGGCGATT
     1801 AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT
     1861 GTAATACGAC TCACTATAGG GCGAATTGGA GCTCCACCGC GGTGGCGGCC GCCAGCTGAA
     1921 GCTTCGTACG CTGCAGGTCG ACGGATCCCC GGGTTAATTA AGGCGCGCCA GATCTCATTA
     1981 TTGCACTGGG TGTTCTGGGC GGGACTGCAC ACGCTGCCAA TGAAGTAACT TTCCTGGGTT
     2041 CAGTAAGCGC AACAACCTGT GACCTGACCA CTTCTGTCAA CGGCGCCGCT CAGCCGAACC
     2101 AGGTTGTTCA GCTGGGTACC GTTCAGGCTA ACCAGCCGGG TAATTTTGTT GACTTCGCCA
     2161 TGAAACCGGT TGACCCGAAT GCACAGGGTT GTGCAAATCT GGCTCAGAAA ACGGCCACTG
     2221 TTTCCTGGGC TTCTGCTGCC CTGGATGGCG AAGGCTTTGG GGCAACCAGC GGTACAGCTA
     2281 CTGATGCCAA AGTACTGGTT GAAAGCGTGA ACTCCAAAAA CCCGGGGGCT GTAAACGCTA
     2341 ACGCCAGCAC GGTTGACTTT GAAGGTGCCA AGCTGACCAC CGACGGCCTG CAGTTCAAAG
     2401 CCAAGCTGAA AGGTGGTGCG ACCGAGTTTA GCTTGCCTCG TCCCCGCCGG GTCACCCGGC
     2461 CAGCGACATG GAGGCCCAGA ATACCCTCCT TGACAGTCTT GACGTGCGCA GCTCAGGGGC
     2521 ATGATGTGAC TGTCGCCCGT ACATTTAGCC CATACATCCC CATGTATAAT CATTTGCATC
     2581 CATACATTTT GATGGCCGCA CGGCGCGAAG CAAAAATTAC GGCTCCTCGC TGCAGACCTG
     2641 CGAGCAGGGA AACGCTCCCC TCACAGACGC GTTGAATTGT CCCCACGCCG CGCCCCTGTA
     2701 GAGAAATATA AAAGGTTAGG ATTTGCCACT GAGGTTCTTC TTTCATATAC TTCCTTTTAA
     2761 AATCTTGCTA GGATACAGTT CTCACATCAC ATCCGAACAT AAACAACCAT GACGTTTGAC
     2821 GACAAAAAAG GTTTACAAGT TGCTCTTGAT CAAGCCAAGA AAAGTATGTG TATATCATGC
     2881 CCACTCTGAA CTTTCAATTA AAATCCAGCT TTACTAACAC ACTTGCTGTC CAGGTTACTC
     2941 AGAAGGTGGG ATACCTATTG GTTCATGTAT TATTTCATCT GATGACACAG TATTAGGCCA
     3001 AGGTCACAAC GAAAGAATCC AAAAACATTC AGCTATTTTA CATGGAGAAA TGTCAGCATT
     3061 AGAAAACGCA GGAAGATTAC CAGGAAAAAC TTATAAAGAT TGTACCATCT ATACTACTTT
     3121 ATCACCATGT AGTATGTGTA CTGGGGCCAT TTTATTATAT GGATTCAAAA GAGTTGTGAT
     3181 GGGAGAAAAT GTCAATTTCT TGGGTAATGA AAAGTTATTG ATTGAAAATG GTGTCGAAGT
     3241 TGTGAATTTA AATGATCAAG AATGTATTGA TTTGATGGCC AAATTCATTA AAGAGAAACC
     3301 ACAAGATTGG AATGAAGATA TTGGAGAATA ATCAGTACTG ACAATAAAAA GATTCTTGTT
     3361 TTCAAGAACT TGTCATTTGT ATAGTTTTTT TATATTGTAG TTGTTCTATT TTAATCAAAT
     3421 GTTAGCGTGA TTTATATTTT TTTTCGCCTC GACATCATCT GCCCAGATGC GAAGTTAAGT
     3481 GCGCAGAAAG TAATATCATG CGTCAATCGT ATGTGAATGC TGGTCGCTAT ACTGCTGTCG
     3541 ATTCGATACT AACGCCGCCA TCCAGTGTCG ACATTATTGC ACTGGGTGTT CTGGGCGGGA
     3601 CTGCACACGC TGCCAATGAA GTAACTTTCC TGGGTTCAGT AAGCGCAACA ACCTGTGACC
     3661 TGACCACTTC TGTCAACGGC GCCGCTCAGC CGAACCAGGT TGTTCAGCTG GGTACCGTTC
     3721 AGGCTAACCA GCCGGGTAAT TTTGTTGACT TCGCCATGAA ACCGGTTGAC CCGAATGCAC
     3781 AGGGTTGTGC AAATCTGGCT CAGAAAACGG CCACTGTTTC CTGGGCTTCT GCTGCCCTGG
     3841 ATGGCGAAGG CTTTGGGGCA ACCAGCGGTA CAGCTACTGA TGCCAAAGTA CTGGTTGAAA
     3901 GCGTGAACTC CAAAAACCCG GGGGCTGTAA ACGCTAACGC CAGCACGGTT GACTTTGAAG
     3961 GTGCCAAGCT GACCACCGAC GGCCTGCAGT TCAAAGCCAA GCTGAAAGGT GGTGCGACCG
     4021 AGTTTAAACG AGCTCGAATT CATCGATGAT ATCAGATCCA CTAGTGGCCT ATGCGGCCGC
     4081 TCTAGAACTA GTGGATCCCC CGGGCTGCAG GAATTCGATA TCAAGCTTAT CGATACCGTC
     4141 GACCTCGAGG GGGGGCCCGG TACCCAGCTT TTGTTCCCTT TAGTGAGGGT TAATTCCGAG
     4201 CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC
     4261 ACACAACATA GGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGGTA
     4321 ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA
     4381 GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC
     4441 CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC
     4501 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT
     4561 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT
     4621 CCATAGGCTC GGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG
     4681 AAACCCGACA GGACTATAAA GATACCAGGC GTTCCCCCCT GGAAGCTCCC TCGTGCGCTC
     4741 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT
     4801 GGCGCTTTCT CAATGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA
     4861 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA
     4921 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA
     4981 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA
     5041 CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT
     5101 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT
     5161 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT
     5221 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT
     5281 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC
     5341 AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC
     5401 ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTGC CCGTCGTGTA
     5461 GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA
     5521 CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG
     5581 CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC
     5641 TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT
     5701 CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG
     5761 GCGAGTTACA TGATCCCCCA TGTTGTGAAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT
     5821 CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA
     5881 TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA
     5941 GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA
     6001 TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG
     6061 GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC
     6121 ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG
     6181 AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT
     6241 CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT
     6301 ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT
     6361 GCCACCTGGG TCCTTTTCAT CACGTGCTAT AAAAATAATT ATAATTTAAA TTTTTTAATA
     6421 TAAATATATA AATTAAAAAT AGAAAGTAAA AAAAGAAATT AAAGAAAAAA TAGTTTTTGT
     6481 TTTCCGAAGA TGTAAAAGAC TCTAGGGGGA TCGCCAACAA ATACTACCTT TTATCTTGCT
     6541 CTTCCTGCTC TCAGGTATTA ATGCCGAATT GTTTCATCTT GTCTGTGTAG AAGACCACAC
     6601 ACGAAAATCC TGTGATTTTA CATTTTACTT ATCGTTAATC GAATGTATAT CTATTTAATC
     6661 TGCTTTTCTT GTCTAATAAA TATATATGTA AAGTACGCTT TTTGTTGAAA TTTTTTAAAC
     6721 CTTTGTTTAT TTTTTTTTCT TCATTCCGTA ACTCTTCTAC CTTCTTTATT TACTTTCTAA
     6781 AATCCAAATA CAAAACATAA AAATAAATAA ACACAGAGTA AATTCCCAAA TTATTCCATC
     6841 ATTAAAAGAT ACGAGGCGCG TGTAAGTTAC AGGCAAGCGA TCCGTCCTAA GAAACCATTA
     6901 TTATCATGAC ATTAACCTAT AAAAATAGGC GTATCACGAG GCCCTTTCGT C
//