LOCUS       PBN53.GB     6905 BP DS-DNA   CIRCULAR  SYN       15-NOV-2006
DEFINITION  Yeast centromere vector pRS314 with TRP1 marker, complete sequence.
ACCESSION   -
KEYWORDS    -
SOURCE      Cloning vector pRS314.
REFERENCE   [1]
  AUTHORS   Sikorski, R.S. & Hieter, P.
  TITLE     A system of shuttle vectors and yeast host strains designed for
            efficient
            manipulation of DNA in Saccharomyces cerevisiae.
  JOURNAL   Genetics,  122,  1,  19-27,  (1989)
  MEDLINE   89276910
REFERENCE   [2]
  AUTHORS   Stillman, D.J.
  TITLE     Direct Submission
  JOURNAL   Submission,  (1993)
COMMENT     DT   14-SEP-1995
            Database Reference:
            GENBANK    PRS314
            Accession:  U03440
            ------------ 
            GenBank Division: SYN
FEATURES             Location/Qualifiers
     gene            468..1142
                     /note="TRP1"
     gene            join(complement(1477..>1908),complement(<4031..>4174))
                     /note="lacZa' [Split]"
     gene            complement(5331..6191)
                     /note="Amp"
     frag            join(1909..>2808,<3286..>4030)
                     /note="2013 to 4467 of pRS316+(pef/Kan/pef).MV [Split]"
     frag            join(1909..>2808,<3286..>4030)
                     /note="9 to 2464 of pFA6-KanMX3 (with pefA DR).MV [Split]"
     primer_bind     1930..1995
                     /note="BN105"
     primer_bind     complement(4007..4028)
                     /note="JM42 rev."
     promoter        2460..>2808
                     /note="P-Tef"
     primer_bind     1913..1931
                     /note="JM41 fwd."
     terminator      3286..3525
                     /note="T-Tef"
     frag            1976..2425
                     /note="24 to 473 of pefA.MV"
     primer_bind     1976..1995
                     /note="BN103"
     primer_bind     complement(2406..2425)
                     /note="BN104"
     repeat_region   1976..2425
                     /note="pefA DR"
     frag            3526..3975
                     /note="24 to 473 of pefA.MV"
     primer_bind     3526..3545
                     /note="BN103"
     primer_bind     complement(3956..3975)
                     /note="BN104"
     repeat_region   3526..3975
                     /note="pefA DR"
     frag            2809..3285
                     /note="2913 to 3389 of pRS316+(pef/FCY1/pef).MV"
     frag            2809..3285
                     /note="459 to 935 of pPH37 (FCY1-MX4)"
     gene            2809..3285
                     /note="FCY1"
BASE COUNT      1845 A      1555 C      1707 G      1798 T         0 OTHER
ORIGIN      Sequence 6905 BP; 1845 A; 1555 C; 1707 G; 1798 T; 0 other;
        1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
       61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
      121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
      181 ACCATAAACG ACATTACTAT ATATATAATA TAGGAAGCAT TTAATAGACA GCATCGTAAT
      241 ATATGTGTAC TTTGCAGTTA TGACGCCAGA TGGCAGTAGT GGAAGATATT CTTTATTGAA
      301 AAATAGCTTG TCACCTTACG TACAATCTTG ATCCGGAGCT TTTCTTTTTT TGCCGATTAA
      361 GAATTAATTC GGTCGAAAAA AGAAAAGGAG AGGGCCAAGA GGGAGGGCAT TGGTGACTAT
      421 TGAGCACGTG AGTATACGTG ATTAAGCACA CAAAGGCAGC TTGGAGTATG TCTGTTATTA
      481 ATTTCACAGG TAGTTCTGGT CCATTGGTGA AAGTTTGCGG CTTGCAGAGC ACAGAGGCCG
      541 CAGAATGTGC TCTAGATTCC GATGCTGACT TGCTGGGTAT TATATGTGTG CCCAATAGAA
      601 AGAGAACAAT TGACCCGGTT ATTGCAAGGA AAATTTCAAG TCTTGTAAAA GCATATAAAA
      661 ATAGTTCAGG CACTCCGAAA TACTTGGTTG GCGTGTTTCG TAATCAACCT AAGGAGGATG
      721 TTTTGGCTCT GGTCAATGAT TACGGCATTG ATATCGTCCA ACTGCATGGA GATGAGTCGT
      781 GGCAAGAATA CCAAGAGTTC CTCGGTTTGC CAGTTATTAA AAGACTCGTA TTTCCAAAAG
      841 ACTGCAACAT ACTACTCAGT GCAGCTTCAC AGAAACCTCA TTCGTTTATT CCCTTGTTTG
      901 ATTCAGAAGC AGGTGGGACA GGTGAACTTT TGGATTGGAA CTCGATTTCT GACTGGGTTG
      961 GAAGGCAAGA GAGCCCCGAA AGCTTACATT TTATGTTAGC TGGTGGACTG ACGCCAGAAA
     1021 ATGTTGGTGA TGCGCTTAGA TTAAATGGCG TTATTGGTGT TGATGTAAGC GGAGGTGTGG
     1081 AGACAAATGG TGTAAAAGAC TCTAACAAAA TAGCAAATTT CGTCAAAAAT GCTAAGAAAT
     1141 AGGTTATTAC TGAGTAGTAT TTATTTAAGT ATTGTTTGTG CACTTGCCTG CGGTGTGAAA
     1201 TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGAA ATTGTAAACG TTAATATTTT
     1261 GTTAAAATTC GCGTTAAATT TTTGTTAAAT CAGCTCATTT TTTAACCAAT AGGCCGAAAT
     1321 CGGCAAAATC CCTTATAAAT CAAAAGAATA GACCGAGATA GGGTTGAGTG TTGTTCCAGT
     1381 TTGGAACAAG AGTCCACTAT TAAAGAACGT GGACTCCAAC GTCAAAGGGC GAAAAACCGT
     1441 CTATCAGGGC GATGGCCCAC TACGTGAACC ATCACCCTAA TCAAGTTTTT TGGGGTCGAG
     1501 GTGCCGTAAA GCACTAAATC GGAACCCTAA AGGGAGCCCC CGATTTAGAG CTTGACGGGG
     1561 AAAGCCGGCG AACGTGGCGA GAAAGGAAGG GAAGAAAGCG AAAGGAGCGG GCGCTAGGGC
     1621 GCTGGCAAGT GTAGCGGTCA CGCTGCGCGT AACCACCACA CCCGCCGCGC TTAATGCGCC
     1681 GCTACAGGGC GCGTCGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC
     1741 GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAGGGG GGATGTGCTG CAAGGCGATT
     1801 AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT
     1861 GTAATACGAC TCACTATAGG GCGAATTGGA GCTCCACCGC GGTGGCGGCC GCCAGCTGAA
     1921 GCTTCGTACG CTGCAGGTCG ACGGATCCCC GGGTTAATTA AGGCGCGCCA GATCTCATTA
     1981 TTGCACTGGG TGTTCTGGGC GGGACTGCAC ACGCTGCCAA TGAAGTAACT TTCCTGGGTT
     2041 CAGTAAGCGC AACAACCTGT GACCTGACCA CTTCTGTCAA CGGCGCCGCT CAGCCGAACC
     2101 AGGTTGTTCA GCTGGGTACC GTTCAGGCTA ACCAGCCGGG TAATTTTGTT GACTTCGCCA
     2161 TGAAACCGGT TGACCCGAAT GCACAGGGTT GTGCAAATCT GGCTCAGAAA ACGGCCACTG
     2221 TTTCCTGGGC TTCTGCTGCC CTGGATGGCG AAGGCTTTGG GGCAACCAGC GGTACAGCTA
     2281 CTGATGCCAA AGTACTGGTT GAAAGCGTGA ACTCCAAAAA CCCGGGGGCT GTAAACGCTA
     2341 ACGCCAGCAC GGTTGACTTT GAAGGTGCCA AGCTGACCAC CGACGGCCTG CAGTTCAAAG
     2401 CCAAGCTGAA AGGTGGTGCG ACCGAGTTTA GCTTGCCTCG TCCCCGCCGG GTCACCCGGC
     2461 CAGCGACATG GAGGCCCAGA ATACCCTCCT TGACAGTCTT GACGTGCGCA GCTCAGGGGC
     2521 ATGATGTGAC TGTCGCCCGT ACATTTAGCC CATACATCCC CATGTATAAT CATTTGCATC
     2581 CATACATTTT GATGGCCGCA CGGCGCGAAG CAAAAATTAC GGCTCCTCGC TGCAGACCTG
     2641 CGAGCAGGGA AACGCTCCCC TCACAGACGC GTTGAATTGT CCCCACGCCG CGCCCCTGTA
     2701 GAGAAATATA AAAGGTTAGG ATTTGCCACT GAGGTTCTTC TTTCATATAC TTCCTTTTAA
     2761 AATCTTGCTA GGATACAGTT CTCACATCAC ATCCGAACAT AAACAACCAT GGTGACAGGG
     2821 GGAATGGCAA GCAAGTGGGA TCAGAAGGGT ATGGACATTG CCTATGAGGA GGCGGCCTTA
     2881 GGTTACAAAG AGGGTGGTGT TCCTATTGGC GGATGTCTTA TCAATAACAA AGACGGAAGT
     2941 GTTCTCGGTC GTGGTCACAA CATGAGATTT CAAAAGGGAT CCGCCACACT ACATGGTGAG
     3001 ATCTCCACTT TGGAAAACTG TGGGAGATTA GAGGGCAAAG TGTACAAAGA TACCACTTTG
     3061 TATACGACGC TGTCTCCATG CGACATGTGT ACAGGTGCCA TCATCATGTA TGGTATTCCA
     3121 CGCTGTGTTG TCGGTGAGAA CGTTAATTTC AAAAGTAAGG GCGAGAAATA TTTACAAACT
     3181 AGAGGTCACG AGGTTGTTGT TGTTGACGAT GAGAGGTGTA AAAAGATCAT GAAACAATTT
     3241 ATCGATGAAA GACCTCAGGA TTGGTTTGAA GATATTGGTG AGTAGTCAGT ACTGACAATA
     3301 AAAAGATTCT TGTTTTCAAG AACTTGTCAT TTGTATAGTT TTTTTATATT GTAGTTGTTC
     3361 TATTTTAATC AAATGTTAGC GTGATTTATA TTTTTTTTCG CCTCGACATC ATCTGCCCAG
     3421 ATGCGAAGTT AAGTGCGCAG AAAGTAATAT CATGCGTCAA TCGTATGTGA ATGCTGGTCG
     3481 CTATACTGCT GTCGATTCGA TACTAACGCC GCCATCCAGT GTCGACATTA TTGCACTGGG
     3541 TGTTCTGGGC GGGACTGCAC ACGCTGCCAA TGAAGTAACT TTCCTGGGTT CAGTAAGCGC
     3601 AACAACCTGT GACCTGACCA CTTCTGTCAA CGGCGCCGCT CAGCCGAACC AGGTTGTTCA
     3661 GCTGGGTACC GTTCAGGCTA ACCAGCCGGG TAATTTTGTT GACTTCGCCA TGAAACCGGT
     3721 TGACCCGAAT GCACAGGGTT GTGCAAATCT GGCTCAGAAA ACGGCCACTG TTTCCTGGGC
     3781 TTCTGCTGCC CTGGATGGCG AAGGCTTTGG GGCAACCAGC GGTACAGCTA CTGATGCCAA
     3841 AGTACTGGTT GAAAGCGTGA ACTCCAAAAA CCCGGGGGCT GTAAACGCTA ACGCCAGCAC
     3901 GGTTGACTTT GAAGGTGCCA AGCTGACCAC CGACGGCCTG CAGTTCAAAG CCAAGCTGAA
     3961 AGGTGGTGCG ACCGAGTTTA AACGAGCTCG AATTCATCGA TGATATCAGA TCCACTAGTG
     4021 GCCTATGCGG CCGCTCTAGA ACTAGTGGAT CCCCCGGGCT GCAGGAATTC GATATCAAGC
     4081 TTATCGATAC CGTCGACCTC GAGGGGGGGC CCGGTACCCA GCTTTTGTTC CCTTTAGTGA
     4141 GGGTTAATTC CGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT
     4201 CCGCTCACAA TTCCACACAA CATAGGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC
     4261 TAATGAGTGA GGTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA
     4321 AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT
     4381 ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG
     4441 CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC
     4501 GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG
     4561 TTGCTGGCGT TTTTCCATAG GCTCGGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA
     4621 AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTCCC CCCTGGAAGC
     4681 TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC
     4741 CCTTCGGGAA GCGTGGCGCT TTCTCAATGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG
     4801 GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC
     4861 TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA
     4921 GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG
     4981 AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG
     5041 AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT
     5101 GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA
     5161 GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA
     5221 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA
     5281 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC
     5341 TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA
     5401 CTGCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA
     5461 ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC
     5521 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT
     5581 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC
     5641 ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT
     5701 TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GAAAAAAAGC GGTTAGCTCC
     5761 TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG
     5821 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT
     5881 GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG
     5941 GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA
     6001 AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG
     6061 TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG
     6121 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT
     6181 TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC
     6241 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA
     6301 TTTCCCCGAA AAGTGCCACC TGGGTCCTTT TCATCACGTG CTATAAAAAT AATTATAATT
     6361 TAAATTTTTT AATATAAATA TATAAATTAA AAATAGAAAG TAAAAAAAGA AATTAAAGAA
     6421 AAAATAGTTT TTGTTTTCCG AAGATGTAAA AGACTCTAGG GGGATCGCCA ACAAATACTA
     6481 CCTTTTATCT TGCTCTTCCT GCTCTCAGGT ATTAATGCCG AATTGTTTCA TCTTGTCTGT
     6541 GTAGAAGACC ACACACGAAA ATCCTGTGAT TTTACATTTT ACTTATCGTT AATCGAATGT
     6601 ATATCTATTT AATCTGCTTT TCTTGTCTAA TAAATATATA TGTAAAGTAC GCTTTTTGTT
     6661 GAAATTTTTT AAACCTTTGT TTATTTTTTT TTCTTCATTC CGTAACTCTT CTACCTTCTT
     6721 TATTTACTTT CTAAAATCCA AATACAAAAC ATAAAAATAA ATAAACACAG AGTAAATTCC
     6781 CAAATTATTC CATCATTAAA AGATACGAGG CGCGTGTAAG TTACAGGCAA GCGATCCGTC
     6841 CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT
     6901 TCGTC
//