LOCUS       PBN62.GB     4558 BP DS-DNA   CIRCULAR  SYN       15-NOV-2006
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
COMMENT     DT   20-JUN-2001
FEATURES             Location/Qualifiers
     gene            complement(3231..4091)
                     /note="Amp"
     gene            909..1431
                     /note="FCA1"
     frag            7..2174
                     /note="1907 to 4074 of pRS314 +(pef/FCA1/pef).MV"
     gene            complement(<7..8)
                     /note="lacZa' [Split]"
     frag            join(9..>908,<1432..>2174)
                     /note="2013 to 4467 of pRS316+(pef/Kan/pef).MV [Split]"
     frag            join(9..>908,<1432..>2174)
                     /note="9 to 2464 of pFA6-KanMX3 (with pefA DR).MV [Split]"
     primer_bind     30..95
                     /note="BN105"
     primer_bind     complement(2153..2174)
                     /note="JM42 rev."
     promoter        560..>908
                     /note="P-Tef"
     primer_bind     13..31
                     /note="JM41 fwd."
     terminator      1432..1671
                     /note="T-Tef"
     frag            76..525
                     /note="24 to 473 of pefA.MV"
     primer_bind     76..95
                     /note="BN103"
     primer_bind     complement(506..525)
                     /note="BN104"
     repeat_region   76..525
                     /note="pefA DR"
     frag            1672..2121
                     /note="24 to 473 of pefA.MV"
     primer_bind     1672..1691
                     /note="BN103"
     primer_bind     complement(2102..2121)
                     /note="BN104"
     repeat_region   1672..2121
                     /note="pefA DR"
     frag            909..1431
                     /note="2913 to 3435 of pRS316+(pef/FCA1/pef).MV"
     frag            909..1431
                     /note="459 to 981 of pPH36 (FCA1-MX4)"
     frag            909..1431
                     /note="858 to 1380 of C. albicans FCA1.MV"
     exon            <909..963
                     /note="exon"
                     /note="/number=1"
     intron          964..1033
                     /note="intron"
     exon            1034..>1431
                     /note="exon"
                     /note="/number=2"
     mRNA            join(<909..963,<1034..>1431)
                     /note="cytosine deaminase"
     CDS             join(909..963,1034..1431)
                     /note="cytosine deaminase"
BASE COUNT      1186 A      1106 C      1109 G      1157 T         0 OTHER
ORIGIN      Sequence 4558 BP; 1186 A; 1106 C; 1109 G; 1157 T; 0 other;
        1 GAACGCGGCC GCCAGCTGAA GCTTCGTACG CTGCAGGTCG ACGGATCCCC GGGTTAATTA
       61 AGGCGCGCCA GATCTCATTA TTGCACTGGG TGTTCTGGGC GGGACTGCAC ACGCTGCCAA
      121 TGAAGTAACT TTCCTGGGTT CAGTAAGCGC AACAACCTGT GACCTGACCA CTTCTGTCAA
      181 CGGCGCCGCT CAGCCGAACC AGGTTGTTCA GCTGGGTACC GTTCAGGCTA ACCAGCCGGG
      241 TAATTTTGTT GACTTCGCCA TGAAACCGGT TGACCCGAAT GCACAGGGTT GTGCAAATCT
      301 GGCTCAGAAA ACGGCCACTG TTTCCTGGGC TTCTGCTGCC CTGGATGGCG AAGGCTTTGG
      361 GGCAACCAGC GGTACAGCTA CTGATGCCAA AGTACTGGTT GAAAGCGTGA ACTCCAAAAA
      421 CCCGGGGGCT GTAAACGCTA ACGCCAGCAC GGTTGACTTT GAAGGTGCCA AGCTGACCAC
      481 CGACGGCCTG CAGTTCAAAG CCAAGCTGAA AGGTGGTGCG ACCGAGTTTA GCTTGCCTCG
      541 TCCCCGCCGG GTCACCCGGC CAGCGACATG GAGGCCCAGA ATACCCTCCT TGACAGTCTT
      601 GACGTGCGCA GCTCAGGGGC ATGATGTGAC TGTCGCCCGT ACATTTAGCC CATACATCCC
      661 CATGTATAAT CATTTGCATC CATACATTTT GATGGCCGCA CGGCGCGAAG CAAAAATTAC
      721 GGCTCCTCGC TGCAGACCTG CGAGCAGGGA AACGCTCCCC TCACAGACGC GTTGAATTGT
      781 CCCCACGCCG CGCCCCTGTA GAGAAATATA AAAGGTTAGG ATTTGCCACT GAGGTTCTTC
      841 TTTCATATAC TTCCTTTTAA AATCTTGCTA GGATACAGTT CTCACATCAC ATCCGAACAT
      901 AAACAACCAT GACGTTTGAC GACAAAAAAG GTTTACAAGT TGCTCTTGAT CAAGCCAAGA
      961 AAAGTATGTG TATATCATGC CCACTCTGAA CTTTCAATTA AAATCCAGCT TTACTAACAC
     1021 ACTTGCTGTC CAGGTTACTC AGAAGGTGGG ATACCTATTG GTTCATGTAT TATTTCATCT
     1081 GATGACACAG TATTAGGCCA AGGTCACAAC GAAAGAATCC AAAAACATTC AGCTATTTTA
     1141 CATGGAGAAA TGTCAGCATT AGAAAACGCA GGAAGATTAC CAGGAAAAAC TTATAAAGAT
     1201 TGTACCATCT ATACTACTTT ATCACCATGT AGTATGTGTA CTGGGGCCAT TTTATTATAT
     1261 GGATTCAAAA GAGTTGTGAT GGGAGAAAAT GTCAATTTCT TGGGTAATGA AAAGTTATTG
     1321 ATTGAAAATG GTGTCGAAGT TGTGAATTTA AATGATCAAG AATGTATTGA TTTGATGGCC
     1381 AAATTCATTA AAGAGAAACC ACAAGATTGG AATGAAGATA TTGGAGAATA ATCAGTACTG
     1441 ACAATAAAAA GATTCTTGTT TTCAAGAACT TGTCATTTGT ATAGTTTTTT TATATTGTAG
     1501 TTGTTCTATT TTAATCAAAT GTTAGCGTGA TTTATATTTT TTTTCGCCTC GACATCATCT
     1561 GCCCAGATGC GAAGTTAAGT GCGCAGAAAG TAATATCATG CGTCAATCGT ATGTGAATGC
     1621 TGGTCGCTAT ACTGCTGTCG ATTCGATACT AACGCCGCCA TCCAGTGTCG ACATTATTGC
     1681 ACTGGGTGTT CTGGGCGGGA CTGCACACGC TGCCAATGAA GTAACTTTCC TGGGTTCAGT
     1741 AAGCGCAACA ACCTGTGACC TGACCACTTC TGTCAACGGC GCCGCTCAGC CGAACCAGGT
     1801 TGTTCAGCTG GGTACCGTTC AGGCTAACCA GCCGGGTAAT TTTGTTGACT TCGCCATGAA
     1861 ACCGGTTGAC CCGAATGCAC AGGGTTGTGC AAATCTGGCT CAGAAAACGG CCACTGTTTC
     1921 CTGGGCTTCT GCTGCCCTGG ATGGCGAAGG CTTTGGGGCA ACCAGCGGTA CAGCTACTGA
     1981 TGCCAAAGTA CTGGTTGAAA GCGTGAACTC CAAAAACCCG GGGGCTGTAA ACGCTAACGC
     2041 CAGCACGGTT GACTTTGAAG GTGCCAAGCT GACCACCGAC GGCCTGCAGT TCAAAGCCAA
     2101 GCTGAAAGGT GGTGCGACCG AGTTTAAACG AGCTCGAATT CATCGATGAT ATCAGATCCA
     2161 CTAGTGGCCT ATGCGGCCGC GGATCTGCCG GTCTCCCTAT AGTGAGTCGT ATTAATTTCG
     2221 ATAAGCCAGG TTAACCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT
     2281 ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG
     2341 CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC
     2401 GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG
     2461 TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA
     2521 AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC
     2581 TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC
     2641 CCTTCGGGAA GCGTGGCGCT TTCTCAATGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG
     2701 GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC
     2761 TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA
     2821 GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG
     2881 AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG
     2941 AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT
     3001 GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA
     3061 GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA
     3121 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA
     3181 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC
     3241 TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA
     3301 CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA
     3361 ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC
     3421 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT
     3481 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC
     3541 ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT
     3601 TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC
     3661 TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG
     3721 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT
     3781 GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG
     3841 GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA
     3901 AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG
     3961 TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG
     4021 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT
     4081 TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC
     4141 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA
     4201 TTTCCCCGAA AAGTGCCACC TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT
     4261 AAAAATAGGC GTATCACGAG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC
     4321 CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC
     4381 AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG GCTTAACTAT
     4441 GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGG ACATATTGTC GTTAGAACGC
     4501 GGCTACAATT AATACATAAC CTTATGTATC ATACACATAC GATTTAGGTG ACACTATA
//