LOCUS       PBN63.GB     4512 BP DS-DNA   CIRCULAR  SYN       15-NOV-2006
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
COMMENT     DT   20-JUN-2001
FEATURES             Location/Qualifiers
     gene            complement(3185..4045)
                     /note="Amp"
     primer_bind     complement(506..565)
                     /note="BN106"
     primer_bind     1586..1645
                     /note="BN107"
     primer_bind     complement(2056..2115)
                     /note="BN108"
     primer_bind     complement(1255..1277)
                     /note="BN200 FCY1 3' rev."
     primer_bind     996..1015
                     /note="BN201 FCY1 5' fwd."
     frag            7..2128
                     /note="1907 to 4028 of pRS314 +(pef/FCY1/pef).MV"
     gene            complement(<7..8)
                     /note="lacZa' [Split]"
     frag            join(9..>908,<1386..>2128)
                     /note="2013 to 4467 of pRS316+(pef/Kan/pef).MV [Split]"
     frag            join(9..>908,<1386..>2128)
                     /note="9 to 2464 of pFA6-KanMX3 (with pefA DR).MV [Split]"
     primer_bind     30..95
                     /note="BN105"
     primer_bind     complement(2107..2128)
                     /note="JM42 rev."
     promoter        560..>908
                     /note="P-Tef"
     primer_bind     13..31
                     /note="JM41 fwd."
     terminator      1386..1625
                     /note="T-Tef"
     frag            76..525
                     /note="24 to 473 of pefA.MV"
     primer_bind     76..95
                     /note="BN103"
     primer_bind     complement(506..525)
                     /note="BN104"
     repeat_region   76..525
                     /note="pefDR"
     frag            1626..2075
                     /note="24 to 473 of pefA.MV"
     primer_bind     1626..1645
                     /note="BN103"
     primer_bind     complement(2056..2075)
                     /note="BN104"
     repeat_region   1626..2075
                     /note="pefDR"
     frag            909..1385
                     /note="2913 to 3389 of pRS316+(pef/FCY1/pef).MV"
     frag            909..1385
                     /note="459 to 935 of pPH37 (FCY1-MX4)"
     gene            909..1385
                     /note="FCY1"
BASE COUNT      1140 A      1104 C      1143 G      1125 T         0 OTHER
ORIGIN      Sequence 4512 BP; 1140 A; 1104 C; 1143 G; 1125 T; 0 other;
        1 GAACGCGGCC GCCAGCTGAA GCTTCGTACG CTGCAGGTCG ACGGATCCCC GGGTTAATTA
       61 AGGCGCGCCA GATCTCATTA TTGCACTGGG TGTTCTGGGC GGGACTGCAC ACGCTGCCAA
      121 TGAAGTAACT TTCCTGGGTT CAGTAAGCGC AACAACCTGT GACCTGACCA CTTCTGTCAA
      181 CGGCGCCGCT CAGCCGAACC AGGTTGTTCA GCTGGGTACC GTTCAGGCTA ACCAGCCGGG
      241 TAATTTTGTT GACTTCGCCA TGAAACCGGT TGACCCGAAT GCACAGGGTT GTGCAAATCT
      301 GGCTCAGAAA ACGGCCACTG TTTCCTGGGC TTCTGCTGCC CTGGATGGCG AAGGCTTTGG
      361 GGCAACCAGC GGTACAGCTA CTGATGCCAA AGTACTGGTT GAAAGCGTGA ACTCCAAAAA
      421 CCCGGGGGCT GTAAACGCTA ACGCCAGCAC GGTTGACTTT GAAGGTGCCA AGCTGACCAC
      481 CGACGGCCTG CAGTTCAAAG CCAAGCTGAA AGGTGGTGCG ACCGAGTTTA GCTTGCCTCG
      541 TCCCCGCCGG GTCACCCGGC CAGCGACATG GAGGCCCAGA ATACCCTCCT TGACAGTCTT
      601 GACGTGCGCA GCTCAGGGGC ATGATGTGAC TGTCGCCCGT ACATTTAGCC CATACATCCC
      661 CATGTATAAT CATTTGCATC CATACATTTT GATGGCCGCA CGGCGCGAAG CAAAAATTAC
      721 GGCTCCTCGC TGCAGACCTG CGAGCAGGGA AACGCTCCCC TCACAGACGC GTTGAATTGT
      781 CCCCACGCCG CGCCCCTGTA GAGAAATATA AAAGGTTAGG ATTTGCCACT GAGGTTCTTC
      841 TTTCATATAC TTCCTTTTAA AATCTTGCTA GGATACAGTT CTCACATCAC ATCCGAACAT
      901 AAACAACCAT GGTGACAGGG GGAATGGCAA GCAAGTGGGA TCAGAAGGGT ATGGACATTG
      961 CCTATGAGGA GGCGGCCTTA GGTTACAAAG AGGGTGGTGT TCCTATTGGC GGATGTCTTA
     1021 TCAATAACAA AGACGGAAGT GTTCTCGGTC GTGGTCACAA CATGAGATTT CAAAAGGGAT
     1081 CCGCCACACT ACATGGTGAG ATCTCCACTT TGGAAAACTG TGGGAGATTA GAGGGCAAAG
     1141 TGTACAAAGA TACCACTTTG TATACGACGC TGTCTCCATG CGACATGTGT ACAGGTGCCA
     1201 TCATCATGTA TGGTATTCCA CGCTGTGTTG TCGGTGAGAA CGTTAATTTC AAAAGTAAGG
     1261 GCGAGAAATA TTTACAAACT AGAGGTCACG AGGTTGTTGT TGTTGACGAT GAGAGGTGTA
     1321 AAAAGATCAT GAAACAATTT ATCGATGAAA GACCTCAGGA TTGGTTTGAA GATATTGGTG
     1381 AGTAGTCAGT ACTGACAATA AAAAGATTCT TGTTTTCAAG AACTTGTCAT TTGTATAGTT
     1441 TTTTTATATT GTAGTTGTTC TATTTTAATC AAATGTTAGC GTGATTTATA TTTTTTTTCG
     1501 CCTCGACATC ATCTGCCCAG ATGCGAAGTT AAGTGCGCAG AAAGTAATAT CATGCGTCAA
     1561 TCGTATGTGA ATGCTGGTCG CTATACTGCT GTCGATTCGA TACTAACGCC GCCATCCAGT
     1621 GTCGACATTA TTGCACTGGG TGTTCTGGGC GGGACTGCAC ACGCTGCCAA TGAAGTAACT
     1681 TTCCTGGGTT CAGTAAGCGC AACAACCTGT GACCTGACCA CTTCTGTCAA CGGCGCCGCT
     1741 CAGCCGAACC AGGTTGTTCA GCTGGGTACC GTTCAGGCTA ACCAGCCGGG TAATTTTGTT
     1801 GACTTCGCCA TGAAACCGGT TGACCCGAAT GCACAGGGTT GTGCAAATCT GGCTCAGAAA
     1861 ACGGCCACTG TTTCCTGGGC TTCTGCTGCC CTGGATGGCG AAGGCTTTGG GGCAACCAGC
     1921 GGTACAGCTA CTGATGCCAA AGTACTGGTT GAAAGCGTGA ACTCCAAAAA CCCGGGGGCT
     1981 GTAAACGCTA ACGCCAGCAC GGTTGACTTT GAAGGTGCCA AGCTGACCAC CGACGGCCTG
     2041 CAGTTCAAAG CCAAGCTGAA AGGTGGTGCG ACCGAGTTTA AACGAGCTCG AATTCATCGA
     2101 TGATATCAGA TCCACTAGTG GCCTATGCGG CCGCGGATCT GCCGGTCTCC CTATAGTGAG
     2161 TCGTATTAAT TTCGATAAGC CAGGTTAACC TGCATTAATG AATCGGCCAA CGCGCGGGGA
     2221 GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG
     2281 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG
     2341 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC
     2401 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA
     2461 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT
     2521 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC
     2581 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA ATGCTCACGC TGTAGGTATC
     2641 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC
     2701 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT
     2761 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG
     2821 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA
     2881 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA
     2941 AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA
     3001 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG
     3061 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC
     3121 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG
     3181 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT
     3241 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG
     3301 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA
     3361 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA
     3421 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC
     3481 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT
     3541 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA
     3601 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT
     3661 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT
     3721 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA
     3781 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG
     3841 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA
     3901 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA
     3961 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG
     4021 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC
     4081 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG
     4141 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC ATTATTATCA
     4201 TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG
     4261 ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG
     4321 CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG
     4381 GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGGACATAT
     4441 TGTCGTTAGA ACGCGGCTAC AATTAATACA TAACCTTATG TATCATACAC ATACGATTTA
     4501 GGTGACACTA TA
//