LOCUS       PBN97.GB     7241 BP DS-DNA   CIRCULAR  SYN       15-NOV-2006
DEFINITION  Yeast centromere vector pRS314 with TRP1 marker, complete sequence.
ACCESSION   -
KEYWORDS    -
SOURCE      Cloning vector pRS314.
REFERENCE   [1]
  AUTHORS   Sikorski, R.S. & Hieter, P.
  TITLE     A system of shuttle vectors and yeast host strains designed for
            efficient
            manipulation of DNA in Saccharomyces cerevisiae.
  JOURNAL   Genetics,  122,  1,  19-27,  (1989)
  MEDLINE   89276910
REFERENCE   [2]
  AUTHORS   Stillman, D.J.
  TITLE     Direct Submission
  JOURNAL   Submission,  (1993)
COMMENT     DT   14-SEP-1995
            Database Reference:
            GENBANK    PRS314
            Accession:  U03440
            ------------ 
            GenBank Division: SYN
FEATURES             Location/Qualifiers
     gene            468..1142
                     /note="TRP1"
     gene            join(complement(1477..>1908),complement(<4367..>4510))
                     /note="lacZa' [Split]"
     gene            complement(5667..6527)
                     /note="Amp"
     frag            <3622..>4366
                     /note="9 to 2464 of pFA6-KanMX3 (with pefA DR).MV [Split]"
     primer_bind     1930..1995
                     /note="BN105"
     primer_bind     complement(4343..4364)
                     /note="JM42 rev."
     promoter        2460..>2808
                     /note="P-Tef"
     primer_bind     1913..1931
                     /note="JM41 fwd."
     terminator      3622..3861
                     /note="T-Tef"
     frag            1976..2425
                     /note="24 to 473 of pefA.MV"
     primer_bind     1976..1995
                     /note="BN103"
     primer_bind     complement(2406..2425)
                     /note="BN104"
     repeat_region   1976..2425
                     /note="pefA DR"
     frag            3862..4311
                     /note="24 to 473 of pefA.MV"
     primer_bind     3862..3881
                     /note="BN103"
     primer_bind     complement(4292..4311)
                     /note="BN104"
     repeat_region   3862..4311
                     /note="pefA DR"
     frag            2809..3621
                     /note="925 to 1737 of pAG61 (CaURA3 MX3).MV"
     frag            <2809..>3621
                     /note="3303 to 3324 of pAG36.MV [Split]"
     gene            2809..3621
                     /note="CaURA3"
BASE COUNT      1989 A      1587 C      1746 G      1919 T         0 OTHER
ORIGIN      Sequence 7241 BP; 1989 A; 1587 C; 1746 G; 1919 T; 0 other;
        1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
       61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
      121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
      181 ACCATAAACG ACATTACTAT ATATATAATA TAGGAAGCAT TTAATAGACA GCATCGTAAT
      241 ATATGTGTAC TTTGCAGTTA TGACGCCAGA TGGCAGTAGT GGAAGATATT CTTTATTGAA
      301 AAATAGCTTG TCACCTTACG TACAATCTTG ATCCGGAGCT TTTCTTTTTT TGCCGATTAA
      361 GAATTAATTC GGTCGAAAAA AGAAAAGGAG AGGGCCAAGA GGGAGGGCAT TGGTGACTAT
      421 TGAGCACGTG AGTATACGTG ATTAAGCACA CAAAGGCAGC TTGGAGTATG TCTGTTATTA
      481 ATTTCACAGG TAGTTCTGGT CCATTGGTGA AAGTTTGCGG CTTGCAGAGC ACAGAGGCCG
      541 CAGAATGTGC TCTAGATTCC GATGCTGACT TGCTGGGTAT TATATGTGTG CCCAATAGAA
      601 AGAGAACAAT TGACCCGGTT ATTGCAAGGA AAATTTCAAG TCTTGTAAAA GCATATAAAA
      661 ATAGTTCAGG CACTCCGAAA TACTTGGTTG GCGTGTTTCG TAATCAACCT AAGGAGGATG
      721 TTTTGGCTCT GGTCAATGAT TACGGCATTG ATATCGTCCA ACTGCATGGA GATGAGTCGT
      781 GGCAAGAATA CCAAGAGTTC CTCGGTTTGC CAGTTATTAA AAGACTCGTA TTTCCAAAAG
      841 ACTGCAACAT ACTACTCAGT GCAGCTTCAC AGAAACCTCA TTCGTTTATT CCCTTGTTTG
      901 ATTCAGAAGC AGGTGGGACA GGTGAACTTT TGGATTGGAA CTCGATTTCT GACTGGGTTG
      961 GAAGGCAAGA GAGCCCCGAA AGCTTACATT TTATGTTAGC TGGTGGACTG ACGCCAGAAA
     1021 ATGTTGGTGA TGCGCTTAGA TTAAATGGCG TTATTGGTGT TGATGTAAGC GGAGGTGTGG
     1081 AGACAAATGG TGTAAAAGAC TCTAACAAAA TAGCAAATTT CGTCAAAAAT GCTAAGAAAT
     1141 AGGTTATTAC TGAGTAGTAT TTATTTAAGT ATTGTTTGTG CACTTGCCTG CGGTGTGAAA
     1201 TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGAA ATTGTAAACG TTAATATTTT
     1261 GTTAAAATTC GCGTTAAATT TTTGTTAAAT CAGCTCATTT TTTAACCAAT AGGCCGAAAT
     1321 CGGCAAAATC CCTTATAAAT CAAAAGAATA GACCGAGATA GGGTTGAGTG TTGTTCCAGT
     1381 TTGGAACAAG AGTCCACTAT TAAAGAACGT GGACTCCAAC GTCAAAGGGC GAAAAACCGT
     1441 CTATCAGGGC GATGGCCCAC TACGTGAACC ATCACCCTAA TCAAGTTTTT TGGGGTCGAG
     1501 GTGCCGTAAA GCACTAAATC GGAACCCTAA AGGGAGCCCC CGATTTAGAG CTTGACGGGG
     1561 AAAGCCGGCG AACGTGGCGA GAAAGGAAGG GAAGAAAGCG AAAGGAGCGG GCGCTAGGGC
     1621 GCTGGCAAGT GTAGCGGTCA CGCTGCGCGT AACCACCACA CCCGCCGCGC TTAATGCGCC
     1681 GCTACAGGGC GCGTCGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC
     1741 GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAGGGG GGATGTGCTG CAAGGCGATT
     1801 AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT
     1861 GTAATACGAC TCACTATAGG GCGAATTGGA GCTCCACCGC GGTGGCGGCC GCCAGCTGAA
     1921 GCTTCGTACG CTGCAGGTCG ACGGATCCCC GGGTTAATTA AGGCGCGCCA GATCTCATTA
     1981 TTGCACTGGG TGTTCTGGGC GGGACTGCAC ACGCTGCCAA TGAAGTAACT TTCCTGGGTT
     2041 CAGTAAGCGC AACAACCTGT GACCTGACCA CTTCTGTCAA CGGCGCCGCT CAGCCGAACC
     2101 AGGTTGTTCA GCTGGGTACC GTTCAGGCTA ACCAGCCGGG TAATTTTGTT GACTTCGCCA
     2161 TGAAACCGGT TGACCCGAAT GCACAGGGTT GTGCAAATCT GGCTCAGAAA ACGGCCACTG
     2221 TTTCCTGGGC TTCTGCTGCC CTGGATGGCG AAGGCTTTGG GGCAACCAGC GGTACAGCTA
     2281 CTGATGCCAA AGTACTGGTT GAAAGCGTGA ACTCCAAAAA CCCGGGGGCT GTAAACGCTA
     2341 ACGCCAGCAC GGTTGACTTT GAAGGTGCCA AGCTGACCAC CGACGGCCTG CAGTTCAAAG
     2401 CCAAGCTGAA AGGTGGTGCG ACCGAGTTTA GCTTGCCTCG TCCCCGCCGG GTCACCCGGC
     2461 CAGCGACATG GAGGCCCAGA ATACCCTCCT TGACAGTCTT GACGTGCGCA GCTCAGGGGC
     2521 ATGATGTGAC TGTCGCCCGT ACATTTAGCC CATACATCCC CATGTATAAT CATTTGCATC
     2581 CATACATTTT GATGGCCGCA CGGCGCGAAG CAAAAATTAC GGCTCCTCGC TGCAGACCTG
     2641 CGAGCAGGGA AACGCTCCCC TCACAGACGC GTTGAATTGT CCCCACGCCG CGCCCCTGTA
     2701 GAGAAATATA AAAGGTTAGG ATTTGCCACT GAGGTTCTTC TTTCATATAC TTCCTTTTAA
     2761 AATCTTGCTA GGATACAGTT CTCACATCAC ATCCGAACAT AAACAACCAT GACAGTCAAC
     2821 ACTAAGACCT ATAGTGAGAG AGCAGAAACT CATGCCTCAC CAGTAGCACA GCGATTATTT
     2881 CGATTAATGG AACTGAAGAA AACCAATTTA TGTGCATCAA TTGACGTTGA TACCACTAAG
     2941 GAATTCCTTG AATTAATTGA TAAATTAGGT CCTTATGTAT GCTTAATCAA GACTCATATT
     3001 GATATAATCA ATGATTTTTC CTATGAATCC ACTATTGAAC CATTATTAGA ACTTTCACGT
     3061 AAACATCAAT TTATGATTTT TGAAGATAGA AAATTTGCTG ATATTGGTAA TACCGTAAAG
     3121 AAACAATATA TTGGTGGAGT TTATAAAATT AGTAGTTGGG CAGATATTAC CAATGCTCAT
     3181 GGTGTCACTG GGAATGGAGT GGTTGAAGGA TTAAAACAGG GAGCTAAAGA AACCACCACC
     3241 AACCAAGAGC CAAGAGGGTT ATTGATGTTA GCTGAATTAT CATCAGTGGG ATCATTAGCA
     3301 TATGGAGAAT ATTCTCAAAA AACTGTTGAA ATTGCTAAAT CCGATAAGGA ATTTGTTATT
     3361 GGATTTATTG CCCAACGTGA TATGGGTGGC CAAGAAGAAG GATTTGATTG GCTTATTATG
     3421 ACACCTGGAG TTGGATTAGA TGATAAAGGT GATGGATTAG GACAACAATA TAGAACTGTT
     3481 GATGAAGTTG TTAGCACTGG AACTGATATT ATCATTGTTG GTAGAGGATT GTTTGGTAAA
     3541 GGAAGAGATC CAGATATTGA AGGTAAAAGG TATAGAAATG CTGGTTGGAA TGCTTATTTG
     3601 AAAAAGACTG GCCAATTATA ATCAGTACTG ACAATAAAAA GATTCTTGTT TTCAAGAACT
     3661 TGTCATTTGT ATAGTTTTTT TATATTGTAG TTGTTCTATT TTAATCAAAT GTTAGCGTGA
     3721 TTTATATTTT TTTTCGCCTC GACATCATCT GCCCAGATGC GAAGTTAAGT GCGCAGAAAG
     3781 TAATATCATG CGTCAATCGT ATGTGAATGC TGGTCGCTAT ACTGCTGTCG ATTCGATACT
     3841 AACGCCGCCA TCCAGTGTCG ACATTATTGC ACTGGGTGTT CTGGGCGGGA CTGCACACGC
     3901 TGCCAATGAA GTAACTTTCC TGGGTTCAGT AAGCGCAACA ACCTGTGACC TGACCACTTC
     3961 TGTCAACGGC GCCGCTCAGC CGAACCAGGT TGTTCAGCTG GGTACCGTTC AGGCTAACCA
     4021 GCCGGGTAAT TTTGTTGACT TCGCCATGAA ACCGGTTGAC CCGAATGCAC AGGGTTGTGC
     4081 AAATCTGGCT CAGAAAACGG CCACTGTTTC CTGGGCTTCT GCTGCCCTGG ATGGCGAAGG
     4141 CTTTGGGGCA ACCAGCGGTA CAGCTACTGA TGCCAAAGTA CTGGTTGAAA GCGTGAACTC
     4201 CAAAAACCCG GGGGCTGTAA ACGCTAACGC CAGCACGGTT GACTTTGAAG GTGCCAAGCT
     4261 GACCACCGAC GGCCTGCAGT TCAAAGCCAA GCTGAAAGGT GGTGCGACCG AGTTTAAACG
     4321 AGCTCGAATT CATCGATGAT ATCAGATCCA CTAGTGGCCT ATGCGGCCGC TCTAGAACTA
     4381 GTGGATCCCC CGGGCTGCAG GAATTCGATA TCAAGCTTAT CGATACCGTC GACCTCGAGG
     4441 GGGGGCCCGG TACCCAGCTT TTGTTCCCTT TAGTGAGGGT TAATTCCGAG CTTGGCGTAA
     4501 TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA
     4561 GGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGGTA ACTCACATTA
     4621 ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA
     4681 TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG
     4741 CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG
     4801 GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA
     4861 GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC
     4921 GGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA
     4981 GGACTATAAA GATACCAGGC GTTCCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG
     5041 ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT
     5101 CAATGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT
     5161 GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG
     5221 TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC
     5281 AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC
     5341 ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA
     5401 GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC
     5461 AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG
     5521 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA
     5581 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT
     5641 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA
     5701 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTGC CCGTCGTGTA GATAACTACG
     5761 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA
     5821 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT
     5881 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT
     5941 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA
     6001 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA
     6061 TGATCCCCCA TGTTGTGAAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA
     6121 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT
     6181 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA
     6241 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG
     6301 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC
     6361 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA
     6421 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT
     6481 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT
     6541 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT
     6601 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGGG
     6661 TCCTTTTCAT CACGTGCTAT AAAAATAATT ATAATTTAAA TTTTTTAATA TAAATATATA
     6721 AATTAAAAAT AGAAAGTAAA AAAAGAAATT AAAGAAAAAA TAGTTTTTGT TTTCCGAAGA
     6781 TGTAAAAGAC TCTAGGGGGA TCGCCAACAA ATACTACCTT TTATCTTGCT CTTCCTGCTC
     6841 TCAGGTATTA ATGCCGAATT GTTTCATCTT GTCTGTGTAG AAGACCACAC ACGAAAATCC
     6901 TGTGATTTTA CATTTTACTT ATCGTTAATC GAATGTATAT CTATTTAATC TGCTTTTCTT
     6961 GTCTAATAAA TATATATGTA AAGTACGCTT TTTGTTGAAA TTTTTTAAAC CTTTGTTTAT
     7021 TTTTTTTTCT TCATTCCGTA ACTCTTCTAC CTTCTTTATT TACTTTCTAA AATCCAAATA
     7081 CAAAACATAA AAATAAATAA ACACAGAGTA AATTCCCAAA TTATTCCATC ATTAAAAGAT
     7141 ACGAGGCGCG TGTAAGTTAC AGGCAAGCGA TCCGTCCTAA GAAACCATTA TTATCATGAC
     7201 ATTAACCTAT AAAAATAGGC GTATCACGAG GCCCTTTCGT C
//