LOCUS       PMKV001.GB   6886 BP DS-DNA   CIRCULAR  SYN       20-JUN-2001
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
FEATURES             Location/Qualifiers
     gene            411..1214
                     /note="URA3"
     primer_bind     complement(4079..<4080)
                     /note="JM42 rev."
     gene            complement(5312..6172)
                     /note="Amp"
     frag            2017..4078
                     /note="1 to 2062 of Untitled1"
     frag            complement(2017..4078)
                     /note="2017 to 4078 of B328 (pMKV001)"
     primer_bind     complement(3609..3669)
                     /note="MV007"
     primer_bind     complement(4060..4078)
                     /note="JM41 fwd."
     primer_bind     >2017..2036
                     /note="JM42 rev. [Split]"
     promoter        complement(>3633..3981)
                     /note="P-Tef"
     terminator      complement(2059..2303)
                     /note="T-Tef"
     frag            complement(2304..3632)
                     /note="1 to 1329 of dsdA"
BASE COUNT      1888 A      1572 C      1650 G      1776 T         0 OTHER
ORIGIN      -
        1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
       61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
      121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
      181 ACCACGCTTT TCAATTCAAT TCATCATTTT TTTTTTATTC TTTTTTTTGA TTTCGGTTTC
      241 TTTGAAATTT TTTTGATTCG GTAATCTCCG AACAGAAGGA AGAACGAAGG AAGGAGCACA
      301 GACTTAGATT GGTATATATA CGCATATGTA GTGTTGAAGA AACATGAAAT TGCCCAGTAT
      361 TCTTAACCCA ACTGCACAGA ACAAAAACCT GCAGGAAACG AAGATAAATC ATGTCGAAAG
      421 CTACATATAA GGAACGTGCT GCTACTCATC CTAGTCCTGT TGCTGCCAAG CTATTTAATA
      481 TCATGCACGA AAAGCAAACA AACTTGTGTG CTTCATTGGA TGTTCGTACC ACCAAGGAAT
      541 TACTGGAGTT AGTTGAAGCA TTAGGTCCCA AAATTTGTTT ACTAAAAACA CATGTGGATA
      601 TCTTGACTGA TTTTTCCATG GAGGGCACAG TTAAGCCGCT AAAGGCATTA TCCGCCAAGT
      661 ACAATTTTTT ACTCTTCGAA GACAGAAAAT TTGCTGACAT TGGTAATACA GTCAAATTGC
      721 AGTACTCTGC GGGTGTATAC AGAATAGCAG AATGGGCAGA CATTACGAAT GCACACGGTG
      781 TGGTGGGCCC AGGTATTGTT AGCGGTTTGA AGCAGGCGGC AGAAGAAGTA ACAAAGGAAC
      841 CTAGAGGCCT TTTGATGTTA GCAGAATTGT CATGCAAGGG CTCCCTATCT ACTGGAGAAT
      901 ATACTAAGGG TACTGTTGAC ATTGCGAAGA GCGACAAAGA TTTTGTTATC GGCTTTATTG
      961 CTCAAAGAGA CATGGGTGGA AGAGATGAAG GTTACGATTG GTTGATTATG ACACCCGGTG
     1021 TGGGTTTAGA TGACAAGGGA GACGCATTGG GTCAACAGTA TAGAACCGTG GATGATGTGG
     1081 TCTCTACAGG ATCTGACATT ATTATTGTTG GAAGAGGACT ATTTGCAAAG GGAAGGGATG
     1141 CTAAGGTAGA GGGTGAACGT TACAGAAAAG CAGGCTGGGA AGCATATTTG AGAAGATGCG
     1201 GCCAGCAAAA CTAAAAAACT GTATTATAAG TAAATGCATG TATACTAAAC TCACAAATTA
     1261 GAGCTTCAAT TTAATTATAT CAGTTATTAC CCTGCGGTGT GAAATACCGC ACAGATGCGT
     1321 AAGGAGAAAA TACCGCATCA GGAAATTGTA AACGTTAATA TTTTGTTAAA ATTCGCGTTA
     1381 AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT
     1441 AAATCAAAAG AATAGACCGA GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA
     1501 CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC
     1561 CCACTACGTG AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA
     1621 AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG
     1681 GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG
     1741 GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCG
     1801 CGCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG
     1861 CTATTACGCC AGCTGGCGAA GGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA
     1921 GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG AATTGTAATA CGACTCACTA
     1981 TAGGGCGAAT TGGAGCTCCA CCGCGGTGGC GGCCGCATAG GCCACTAGTG GATCTGATAT
     2041 CATCGATGAA TTCGAGCTCG TTTTCGACAC TGGATGGCGG CGTTAGTATC GAATCGACAG
     2101 CAGTATAGCG ACCAGCATTC ACATACGATT GACGCATGAT ATTACTTTCT GCGCACTTAA
     2161 CTTCGCATCT GGGCAGATGA TGTCGAGGCG AAAAAAAATA TAAATCACGC TAACATTTGA
     2221 TTAAAATAGA ACAACTACAA TATAAAAAAA CTATACAAAT GACAAGTTCT TGAAAACAAG
     2281 AATCTTTTTA TTGTCAGTAC TGATTAACGG CCTTTTGCCA GATATTGATT CATCTCTTCT
     2341 TCCGGCACCA TTCCACCTCC CGTCGCCCAC ACCAGATGAG TGGTATTACG CAGTTGTTCT
     2401 GCGCTGAAAC CGTGCATCTG TTGGTAACTT ACTGATGCAC ACACGCGCTG AGGTCCGGCC
     2461 ATACCCGCCA GTGCCGAAGG TTCAAGACGA ATACCTTCTT CCTGCGCCAG CCAGCCAAGC
     2521 ATGTCATACA TGGTTTGATC GCTAAGGGTA TAGAAGCCAT CCAGCAGACG CTCCATTGCC
     2581 CGCCCGACAA AGCCTGATGC GCGACCAACT GCAAGGCCAT CCGCTGCGGT AAGGTTGTCG
     2641 ATACCAATAT CCTGAACAGA AATCTGATCG TGTAATCCTG TATGGACGCC TAACAACATA
     2701 CAAGGGGAGT GCGTTGGTTC GGCAAAAAAG CAGTGAACAT GATCGCCAAA CGCCAGTTTA
     2761 AGCCCGAATG CGACGCCACC AGGACCACCG CCAACACCAC ACGGCAGATA GACAAACAGA
     2821 GGGTTATCAG CATCGACGAT ACGGCCTTGC TGGGCAAATT GCGCTTTAAG ACGCTGGCCA
     2881 GCGACGGAAT ACCCAAGGAA CAACGTGCGG GAATTTTCGT CATCAATAAA GAAACAGTTC
     2941 GGGTCAGACT GCGCTGCTTT ACGTCCTTCC TCGACGGCAA CACCATAATC TTGCTCATAT
     3001 TCCACGACCG TAACGCCATG GCTGCGCAGT TTCGCTTTTT TCCATGCCCG GGCATCAGCA
     3061 GACATATGAA CTGTCACCTT AAAGCCAATG CGGGCGCTCA TAATGCCGAT TGATAACCCC
     3121 AGATTTCCGG TTGAGCCCAC AGCAATGCTG TATTGGCTAA AGAACTGTTT AAACTCCGGA
     3181 GAAAGCAGTT TGCTGTAGTC ATCATCAAGC GTCAGCAACC CCGCTTCCAG AGCCAGTTTT
     3241 TCTGCGTGTG CCAGGACTTC ATAAATCCCG CCGCGTGCTT TTATGGAGCC GGAAATGGGC
     3301 AAATGGCTAT CTTTTTTCAG TAACAGTTGC CCGCTGATCG GTTGCTGATA TTCTTTTTCC
     3361 AGCCGTTTTT GCATAGCTGG AATGGCAACC AGTTCTGATT CAATAATCCC CCCAGTGGCA
     3421 GCAGTTTCAG GAAATGCTTT TGCCAGATAG GGTGCAAAAC GGGATAAGCG CGCATGGGCG
     3481 TCCTGAACAT CCTGTTCGGT CAGGCCAACA TAAGGTAAAC CTTCAGCCAA TGAGGTCGTG
     3541 CCAGGATTAA ACCAGGTGGT TTCTTTAAGA GCAACCAGAT CCTTTACCAA CGGATACTGG
     3601 GCGATGAGCG AGTTCATTTT AGCGTTTTCC ATGGTTGTTT ATGTTCGGAT GTGATGTGAG
     3661 AACTGTATCC TAGCAAGATT TTAAAAGGAA GTATATGAAA GAAGAACCTC AGTGGCAAAT
     3721 CCTAACCTTT TATATTTCTC TACAGGGGCG CGGCGTGGGG ACAATTCAAC GCGTCTGTGA
     3781 GGGGAGCGTT TCCCTGCTCG CAGGTCTGCA GCGAGGAGCC GTAATTTTTG CTTCGCGCCG
     3841 TGCGGCCATC AAAATGTATG GATGCAAATG ATTATACATG GGGATGTATG GGCTAAATGT
     3901 ACGGGCGACA GTCACATCAT GCCCCTGAGC TGCGCACGTC AAGACTGTCA AGGAGGGTAT
     3961 TCTGGGCCTC CATGTCGCTG GCCGGGTGAC CCGGCGGGGA CGAGGCAAGC TAAACAGATC
     4021 TGGCGCGCCT TAATTAACCC GGGGATCCGT CGACCTGCAG CGTACGAAGC TTCAGCTGGC
     4081 GGCCGCCCGC TCTAGTACCC AGCTTTTGTT CCCTTTAGTG AGGGTTAATT CCGAGCTTGG
     4141 CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA
     4201 ACATAGGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGGTAACTCA
     4261 CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC
     4321 ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT
     4381 CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT
     4441 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG
     4501 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA
     4561 GGCTCGGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
     4621 CGACAGGACT ATAAAGATAC CAGGCGTTCC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
     4681 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
     4741 TTTCTCAATG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
     4801 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
     4861 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
     4921 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
     4981 GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
     5041 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
     5101 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT
     5161 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT
     5221 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT
     5281 AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA
     5341 TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTGCCCGTC GTGTAGATAA
     5401 CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC
     5461 GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA
     5521 GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG
     5581 TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG
     5641 TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG
     5701 TTACATGATC CCCCATGTTG TGAAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG
     5761 TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC
     5821 TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT
     5881 TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA
     5941 CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA
     6001 AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA
     6061 ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC
     6121 AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC
     6181 TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
     6241 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
     6301 CTGGGTCCTT TTCATCACGT GCTATAAAAA TAATTATAAT TTAAATTTTT TAATATAAAT
     6361 ATATAAATTA AAAATAGAAA GTAAAAAAAG AAATTAAAGA AAAAATAGTT TTTGTTTTCC
     6421 GAAGATGTAA AAGACTCTAG GGGGATCGCC AACAAATACT ACCTTTTATC TTGCTCTTCC
     6481 TGCTCTCAGG TATTAATGCC GAATTGTTTC ATCTTGTCTG TGTAGAAGAC CACACACGAA
     6541 AATCCTGTGA TTTTACATTT TACTTATCGT TAATCGAATG TATATCTATT TAATCTGCTT
     6601 TTCTTGTCTA ATAAATATAT ATGTAAAGTA CGCTTTTTGT TGAAATTTTT TAAACCTTTG
     6661 TTTATTTTTT TTTCTTCATT CCGTAACTCT TCTACCTTCT TTATTTACTT TCTAAAATCC
     6721 AAATACAAAA CATAAAAATA AATAAACACA GAGTAAATTC CCAAATTATT CCATCATTAA
     6781 AAGATACGAG GCGCGTGTAA GTTACAGGCA AGCGATCCGT CCTAAGAAAC CATTATTATC
     6841 ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTC
//