LOCUS       PMKV006.GB   6954 BP DS-DNA   CIRCULAR  SYN       20-JUN-2001
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
FEATURES             Location/Qualifiers
     gene            411..1214
                     /note="URA3"
     misc_feature    6376..6511
                     /note="cen6"
     gene            complement(5380..6240)
                     /note="Amp"
     repeat_unit     4083..4116
                     /note="loxP"
     repeat_unit     2057..2090
                     /note="loxP"
     gene            2489..3817
                     /note="dsdA"
     promoter        <2140..>2488
                     /note="P-Tef"
     terminator      3818..>4062
                     /note="T-Tef"
BASE COUNT      1872 A      1563 C      1671 G      1848 T         0 OTHER
ORIGIN      -
        1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
       61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
      121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
      181 ACCACGCTTT TCAATTCAAT TCATCATTTT TTTTTTATTC TTTTTTTTGA TTTCGGTTTC
      241 TTTGAAATTT TTTTGATTCG GTAATCTCCG AACAGAAGGA AGAACGAAGG AAGGAGCACA
      301 GACTTAGATT GGTATATATA CGCATATGTA GTGTTGAAGA AACATGAAAT TGCCCAGTAT
      361 TCTTAACCCA ACTGCACAGA ACAAAAACCT GCAGGAAACG AAGATAAATC ATGTCGAAAG
      421 CTACATATAA GGAACGTGCT GCTACTCATC CTAGTCCTGT TGCTGCCAAG CTATTTAATA
      481 TCATGCACGA AAAGCAAACA AACTTGTGTG CTTCATTGGA TGTTCGTACC ACCAAGGAAT
      541 TACTGGAGTT AGTTGAAGCA TTAGGTCCCA AAATTTGTTT ACTAAAAACA CATGTGGATA
      601 TCTTGACTGA TTTTTCCATG GAGGGCACAG TTAAGCCGCT AAAGGCATTA TCCGCCAAGT
      661 ACAATTTTTT ACTCTTCGAA GACAGAAAAT TTGCTGACAT TGGTAATACA GTCAAATTGC
      721 AGTACTCTGC GGGTGTATAC AGAATAGCAG AATGGGCAGA CATTACGAAT GCACACGGTG
      781 TGGTGGGCCC AGGTATTGTT AGCGGTTTGA AGCAGGCGGC AGAAGAAGTA ACAAAGGAAC
      841 CTAGAGGCCT TTTGATGTTA GCAGAATTGT CATGCAAGGG CTCCCTATCT ACTGGAGAAT
      901 ATACTAAGGG TACTGTTGAC ATTGCGAAGA GCGACAAAGA TTTTGTTATC GGCTTTATTG
      961 CTCAAAGAGA CATGGGTGGA AGAGATGAAG GTTACGATTG GTTGATTATG ACACCCGGTG
     1021 TGGGTTTAGA TGACAAGGGA GACGCATTGG GTCAACAGTA TAGAACCGTG GATGATGTGG
     1081 TCTCTACAGG ATCTGACATT ATTATTGTTG GAAGAGGACT ATTTGCAAAG GGAAGGGATG
     1141 CTAAGGTAGA GGGTGAACGT TACAGAAAAG CAGGCTGGGA AGCATATTTG AGAAGATGCG
     1201 GCCAGCAAAA CTAAAAAACT GTATTATAAG TAAATGCATG TATACTAAAC TCACAAATTA
     1261 GAGCTTCAAT TTAATTATAT CAGTTATTAC CCTGCGGTGT GAAATACCGC ACAGATGCGT
     1321 AAGGAGAAAA TACCGCATCA GGAAATTGTA AACGTTAATA TTTTGTTAAA ATTCGCGTTA
     1381 AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT
     1441 AAATCAAAAG AATAGACCGA GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA
     1501 CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC
     1561 CCACTACGTG AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA
     1621 AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG
     1681 GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG
     1741 GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCG
     1801 CGCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG
     1861 CTATTACGCC AGCTGGCGAA GGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA
     1921 GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG AATTGTAATA CGACTCACTA
     1981 TAGGGCGAAT TGGAGCTCCA CCGCGGTGGC GGCCGCCAGC TGAAGCTTCG TACGCTGCAG
     2041 GTCGACAACC CTTAATATAA CTTCGTATAA TGTATGCTAT ACGAAGTTAT TAGGTCTAGA
     2101 GATCTGTTTA GCTTGCCTCG TCCCCGCCGG GTCACCCGGC CAGCGACATG GAGGCCCAGA
     2161 ATACCCTCCT TGACAGTCTT GACGTGCGCA GCTCAGGGGC ATGATGTGAC TGTCGCCCGT
     2221 ACATTTAGCC CATACATCCC CATGTATAAT CATTTGCATC CATACATTTT GATGGCCGCA
     2281 CGGCGCGAAG CAAAAATTAC GGCTCCTCGC TGCAGACCTG CGAGCAGGGA AACGCTCCCC
     2341 TCACAGACGC GTTGAATTGT CCCCACGCCG CGCCCCTGTA GAGAAATATA AAAGGTTAGG
     2401 ATTTGCCACT GAGGTTCTTC TTTCATATAC TTCCTTTTAA AATCTTGCTA GGATACAGTT
     2461 CTCACATCAC ATCCGAACAT AAACAACCAT GGAAAACGCT AAAATGAACT CGCTCATCGC
     2521 CCAGTATCCG TTGGTAAAGG ATCTGGTTGC TCTTAAAGAA ACCACCTGGT TTAATCCTGG
     2581 CACGACCTCA TTGGCTGAAG GTTTACCTTA TGTTGGCCTG ACCGAACAGG ATGTTCAGGA
     2641 CGCCCATGCG CGCTTATCCC GTTTTGCACC CTATCTGGCA AAAGCATTTC CTGAAACTGC
     2701 TGCCACTGGG GGGATTATTG AATCAGAACT GGTTGCCATT CCAGCTATGC AAAAACGGCT
     2761 GGAAAAAGAA TATCAGCAAC CGATCAGCGG GCAACTGTTA CTGAAAAAAG ATAGCCATTT
     2821 GCCCATTTCC GGCTCCATAA AAGCACGCGG CGGGATTTAT GAAGTCCTGG CACACGCAGA
     2881 AAAACTGGCT CTGGAAGCGG GGTTGCTGAC GCTTGATGAT GACTACAGCA AACTGCTTTC
     2941 TCCGGAGTTT AAACAGTTCT TTAGCCAATA CAGCATTGCT GTGGGCTCAA CCGGAAATCT
     3001 GGGGTTATCA ATCGGCATTA TGAGCGCCCG CATTGGCTTT AAGGTGACAG TTCATATGTC
     3061 TGCTGATGCC CGGGCATGGA AAAAAGCGAA ACTGCGCAGC CATGGCGTTA CGGTCGTGGA
     3121 ATATGAGCAA GATTATGGTG TTGCCGTCGA GGAAGGACGT AAAGCAGCGC AGTCTGACCC
     3181 GAACTGTTTC TTTATTGATG ACGAAAATTC CCGCACGTTG TTCCTTGGGT ATTCCGTCGC
     3241 TGGCCAGCGT CTTAAAGCGC AATTTGCCCA GCAAGGCCGT ATCGTCGATG CTGATAACCC
     3301 TCTGTTTGTC TATCTGCCGT GTGGTGTTGG CGGTGGTCCT GGTGGCGTCG CATTCGGGCT
     3361 TAAACTGGCG TTTGGCGATC ATGTTCACTG CTTTTTTGCC GAACCAACGC ACTCCCCTTG
     3421 TATGTTGTTA GGCGTCCATA CAGGATTACA CGATCAGATT TCTGTTCAGG ATATTGGTAT
     3481 CGACAACCTT ACCGCAGCGG ATGGCCTTGC AGTTGGTCGC GCATCAGGCT TTGTCGGGCG
     3541 GGCAATGGAG CGTCTGCTGG ATGGCTTCTA TACCCTTAGC GATCAAACCA TGTATGACAT
     3601 GCTTGGCTGG CTGGCGCAGG AAGAAGGTAT TCGTCTTGAA CCTTCGGCAC TGGCGGGTAT
     3661 GGCCGGACCT CAGCGCGTGT GTGCATCAGT AAGTTACCAA CAGATGCACG GTTTCAGCGC
     3721 AGAACAACTG CGTAATACCA CTCATCTGGT GTGGGCGACG GGAGGTGGAA TGGTGCCGGA
     3781 AGAAGAGATG AATCAATATC TGGCAAAAGG CCGTTAATCA GTACTGACAA TAAAAAGATT
     3841 CTTGTTTTCA AGAACTTGTC ATTTGTATAG TTTTTTTATA TTGTAGTTGT TCTATTTTAA
     3901 TCAAATGTTA GCGTGATTTA TATTTTTTTT CGCCTCGACA TCATCTGCCC AGATGCGAAG
     3961 TTAAGTGCGC AGAAAGTAAT ATCATGCGTC AATCGTATGT GAATGCTGGT CGCTATACTG
     4021 CTGTCGATTC GATACTAACG CCGCCATCCA GTGTCGAAAA CGAGCTCTCG AGAACCCTTA
     4081 ATATAACTTC GTATAATGTA TGCTATACGA AGTTATTAGG TGATATCAGA TCCACTAGTG
     4141 GCCTATGCGG CCGCCCGCTC TAGTACCCAG CTTTTGTTCC CTTTAGTGAG GGTTAATTCC
     4201 GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT
     4261 TCCACACAAC ATAGGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG
     4321 GTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG
     4381 CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC
     4441 TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC
     4501 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA
     4561 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT
     4621 TTTCCATAGG CTCGGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG
     4681 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTCCCC CCTGGAAGCT CCCTCGTGCG
     4741 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG
     4801 CGTGGCGCTT TCTCAATGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC
     4861 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA
     4921 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG
     4981 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC
     5041 TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC
     5101 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG
     5161 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT
     5221 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT
     5281 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA
     5341 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA
     5401 GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TGCCCGTCGT
     5461 GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG
     5521 AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA
     5581 GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA
     5641 AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG
     5701 CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC
     5761 AAGGCGAGTT ACATGATCCC CCATGTTGTG AAAAAAAGCG GTTAGCTCCT TCGGTCCTCC
     5821 GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA
     5881 TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC
     5941 CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG
     6001 GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC
     6061 GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG
     6121 TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC
     6181 AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT
     6241 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA
     6301 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA
     6361 AGTGCCACCT GGGTCCTTTT CATCACGTGC TATAAAAATA ATTATAATTT AAATTTTTTA
     6421 ATATAAATAT ATAAATTAAA AATAGAAAGT AAAAAAAGAA ATTAAAGAAA AAATAGTTTT
     6481 TGTTTTCCGA AGATGTAAAA GACTCTAGGG GGATCGCCAA CAAATACTAC CTTTTATCTT
     6541 GCTCTTCCTG CTCTCAGGTA TTAATGCCGA ATTGTTTCAT CTTGTCTGTG TAGAAGACCA
     6601 CACACGAAAA TCCTGTGATT TTACATTTTA CTTATCGTTA ATCGAATGTA TATCTATTTA
     6661 ATCTGCTTTT CTTGTCTAAT AAATATATAT GTAAAGTACG CTTTTTGTTG AAATTTTTTA
     6721 AACCTTTGTT TATTTTTTTT TCTTCATTCC GTAACTCTTC TACCTTCTTT ATTTACTTTC
     6781 TAAAATCCAA ATACAAAACA TAAAAATAAA TAAACACAGA GTAAATTCCC AAATTATTCC
     6841 ATCATTAAAA GATACGAGGC GCGTGTAAGT TACAGGCAAG CGATCCGTCC TAAGAAACCA
     6901 TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTC
//