Orthopaedic Research Laboratories

Duke University Medical Center

Farshid Guilak, Ph.D., Director of Research


 

Frequently asked questions on adult cells derived from fat becoming cartilage, bone, or fat cells:

 

Q:  When will this be available for patients?  How can I volunteer for medical studies on this technique?

A:  We are many years away from testing this in patients.  We still have significantly laboratory work remaining to show the efficacy and the safety of this procedure.  We will not be seeking volunteers for studies for several years.

 

Q:  Where did you get the fat cells?

A:  They were isolated from fat taken from liposuction procedures for cosmetic surgery.  The liposuction material was digested and the fat cells were separated from the stromal cells that also reside in fat tissue.  We discard the fat cells and use the stromal cells for our experiments. In another experiment, we used a small piece of fat that is found behind the kneecap.

 

Q:  How did you convert fat cells to cartilage cells?

A:   The cells were placed in a gelatinous material (alginate) that caused them take a rounded shape.  Then they were treated with a mixture of chemicals that cause them to increase their production of cartilage-specific molecules.  These chemicals included transformation growth factor beta, dexamethasone, and vitamin C.

 

Q:  Where can I find more information on this procedure?

A:  There is some more information in the Duke University News Office.  You may also request reprints of our scientific publications by sending email to farshid.guilak at duke.edu.

 

Q:  Has this work been published in the scientific literature?

A:  Yes, we have published a paper on the conversion of fat stem cells to cartilage in the journal Biochemical and Biophysical Research Communications.  Also, abstracts of this work were published at the annual meeting of the Orthopaedic Research Society   Please send an email to farshid.guilak@duke.edu to request reprints.

 

Q:  I have arthritis or other joint disease - will this be a possible treatment for me?

A:  We don't foresee using this technique in the near future to treat arthritis.  In many cases, joint replacement may be an option.  This type of surgery is performed in the Division of Orthopaedic Surgery by highly experienced orthopaedic surgeons. You can read more about total joint replacement surgery.

 

 


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