Mouse Genotyping Resources

 

 

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Mouse Genes and Mutations:

Gene/Mutation Primer ID PCR product size (kB)
  common mutant wildtype mutant wildtype
E47bm YZ-104 YZ-29 YZ-164 2.0 1.7
E2Agal YZ-177 YZ-24 E19 0.9 1.1
E2Aflox (WT / flox) YZ-104 YZ-150 YZ-164 2.0 1.8
E2Aflox (flox / del) YZ-198 PGKneo for E2Aflox for 0.5 (del) 1.2 (flox)
HEBko YZ-119 YZ-29 YZ-122 2.8 2.2
Id3ko YZ-151 YZ-29 YZ-170 2.5 2.1
E2AGFP YZ-104 YZ-29 YZ-164 2.0 1.7
LckCre Lck-p Cre3 0.35
HEBbm** YZ-199 YZ-122   1.8 2.0
Mx-Cretg MxCre5 MxCre3   0.5  
CD4Cre Cre5 Cre3   0.35  
HEBflox (flox/wt) JW1 JW2   1.3 1.1
HEBflox (flox/del) JW1 JW2   0.5 (del) 1.3 (flox)
E2Adel E2Aflox for YZ198 E19 0.5 1.1
TCR Vb8.2CD2 P1 P3 P2 1.1 1.8
E2AFH YZ-104 YZ-29 YZ-164 2.0 1.7

**For HEBbm genotyping, digest with NcoI after PCR to detect mutant vs. wt alleles

Genotyping PCR Protocol

Quantitative PCR analysis of E2A deletion

Genotyping Primers

YZ-24            5'-GTT GTG CCC AGT CAT AGC CG-3'                                                Neo antisense

YZ-29            5'-TCG CAG CGC ATC GCC TTC TA-3'                                                Neo sense

YZ-104          5'-ATG TGT GGT GGC CCA CAC TTG T-3'                                          E2A antisense

YZ-119          5'-GAC ATC AAG GTC TCA TCT AGG-3'                                             HEB

YZ-122          5'-TCT CAC TTG CTG TTC TAG ACT-3'

YZ-150          5'-ACA TGG CTG AAT ATC GAC GGT-3'                                            βgal sense

YZ-151          5'-GTT TTG AAC ATA GGT CTG CC-3'

YZ-164          5'-AAG AAC GAG GCC TTC CGT GTC-3'                                            E2A sense

YZ-170          5'-CAC CGG GCT CAG CGC CTT CAT-3'

YZ-177          5'-TTG TGG ACA TTT TCT AGG CAG-3'                                              E2A sense

YZ-198          5'-CGG ATC CAT CCT CGT CTT CAT TGG TAC TG-3'                     

YZ-199          5'-ATG TGA AGC TTG GGG ATT TTG-3'

E19                5'-CCG AGC TCC TTA AAG GCC TCA-3'                                            E12 antisense

Lck-p             5'-GCA GGA AGT GGG TAA CTA GAC TAA C-3'                              Lck promoter

Cre5              5'-CGC AGA ACC TGA AGA TGT TCG CGA TTA-3'                          Cre sense

Cre3              5'-TCT CCC ACC GTC AGT ACG TGA GAT ATC-3'                           Cre antisense

E2Aflox for    5'-CTG CAC TCC GAA TTG TGC CTG-3'                                            E2A sense (5' of loxP)

PGKneo for   5'-GCC CAT TCG ACC ACC AAG CG-3'                                               Neo ORF forward

MxCre5         5'-GTG AGT TTC GTT TCT GAG CTC C-3'                                          Mx promoter

MxCre3         5'-taa tcg cga aca tct tca ggt tct gcg-3'                           Cre ORF

JW1               5'-CTG GGA CAG AAG TTC AGC ACT TAG TAC-3'                          HEB sense

JW2               5'-CAT TCC TAT ACA TCA GCT TCT TGG ACG-3'                            HEB antisense

Vb8.2 P1       5'-ATG GTG CTG GCA GCA CTG AG-3'                                               TCR Vb sense

Vb8.2 P2       5'-CCG GAA TTC AGG GAT GTT GTG TCA TAT TAT GAT GC-3'   TCR Vb antisense

Vb8.2 P3       5'-CCT GAT CAT CGG TCT TCA GAT GC-3'                                       hCD2 antisense

 

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